摘要
目的 探讨脂蛋白脂酶(LPL)基因中常见多态位点HindⅢ基因多态性与儿童单纯肥胖之间的关系。方法 用聚合酶链反应(PCR)和限制性内切酶片段长度多态性(RFLP)技术,检测92 例单纯肥胖儿童和80 例正常儿童的HindⅢ-LPL基因多态性,测定血脂、脂蛋白含量及体块指数(BMI)、血压、皮褶厚度等。结果 肥胖组H+ H+ -LPL基因型频率及H+ 等位基因频率分别为59.78% 和79.35% ,明显高于正常对照组。肥胖组H+ H+ -LPL基因型亚组的TG、TC、LDL-C、ApoB含量以及BMI、舒张压、肱二头肌皮褶厚度、肩胛下皮褶厚度等,均明显高于H+ H- -LPL基因型亚组。结论 H+ H+ 与儿童单纯肥胖相关,等位基因H+
Objective To study the relationship between Hind Ⅲ DNA polymorphism in the lipoprotein lipase(LPL)gene and children simple obesity. Methods The polymerase chain reaction(PCR)and restriction fragment length polymorphism(RFLP)technique was used to detect the LPL Hind Ⅲ polymorphism in 92 children with simple obesity and 80 healthy controls.The levels of the plasma lipid,plasma lipoproteins,body mass index(BMI),blood pressure,skinfolds sickness were also measured. Results The prevalence of genotype H +H + and the frequence of allele H + were 59.78% and 79.35%,respectively,in the obesity group,which were significantly higher than those( 42.50% and 69.38% ,respectively) in the control group( P <0.05).The levels of TG,TC,LDL C,Apo B,BMI,diastolic pressure,biceps and subscapular skinfolds sickness were also significantly higher in the obesity group with H +H +genotype than those in the obesity group with H +H - genotype. Conclusion Genotype of H +H + may contribute to the development of children simple obesity and allele H + seems to be a genetic susceptible marker in children simple obesity.
出处
《实用儿科临床杂志》
CAS
CSCD
1999年第6期316-318,共3页
Journal of Applied Clinical Pediatrics
关键词
脂蛋白脂酶
基因多态性
肥胖
儿童
lipoprotein lipase
polymorphism
obesity
children
