摘要
采用分离提取，电泳回收，ＰＡＧＥ，ＳＤＳ－ＰＡＧＥ，亲和电泳和抑制活性测定等方法，对我国一年生和多年生野生大豆种子蛋白中的胰蛋白酶抑制剂（ＳＢＴｉ）进行了初步研究．在４５°Ｎ 来源的一年生野生大豆（Ｇ．ｓｏｊａ）中得到纯化的胰蛋白酶抑制剂．通过电泳迁移率、分子质量、末端分析和抑制活性等测定，证明为ＳＢＴｉ－Ａ２的Ｔｉｂ型．从我国２４°Ｎ 的２种多年生野生大豆多毛豆（Ｇ．ｔｏｍ ｅｎｔｅｌｌａ）和胭豆（Ｇ．ｔａｂａｃｉｎａ）种子蛋白中发现存在ＳＢＴｉ蛋白．ＰＡＧＥ显示各有３条谱带；亲和电泳证明这３条带均能与胰蛋白酶相结合；ＢＡＥＥ测定法证明它们均具有抑制胰蛋白酶的活性；经ＳＤＳ－ＰＡＧＥ电泳发现这３条带均能各自再分离为２条带，且彼此分子质量相等．这些研究结果表现为胰蛋白酶同工抑制剂性质。
A preliminary study on soybean trypsin inhibitor (SBTi) extracted from annual wild soybean (G.soja) and perennial wild soybean (G.tomentella, G. tabacina) was reported. From the seeds of 45° N G.soja, a purified SBTi was obtained and proved to belong to Ti b type of SBTi A2 through electrophoresis, molecular mass N terminal residues and trypsin inhibiting activity methods. It has been found that SBTi protein existed in both seeds of 24° N G.tomentella and G. tabacina, it was also found that SBTi has three protein bands which could combine with trypsin by PAGE,and have trypsin inhibitory activity by BAEE method. The three bands could be separated into two bands respectively by SDS PAGE. It reveals that the biochemical character of SBTi in perennial wild soybean was quite different from the annual wild one.
出处
《复旦学报（自然科学版）》
CAS
CSCD
北大核心
1999年第5期529-532,共4页
Journal of Fudan University：Natural Science
基金
国家自然科学基金
关键词
一年生
野生
大豆
多年生
胰蛋白酶抑制剂
annual wild soybean
perennial wild soybean
trypsin inhibitor
isoinhibitor
