摘要
目的探讨失交感神经支配对大鼠下颌骨牵张成骨新骨形成的影响。方法雄性SD大鼠30只,随机分为2组,对照组(A组,15只)实施右侧下颌骨牵张成骨,实验组(B组,15只)实施双侧颈交感干离断及右侧下颌骨牵张成骨。术后在固定期1、14、28天取材,对牵张间隙新生骨痂进行大体形态及HE染色骨组织定量观察。应用SPSS12.0软件包进行两样本t检验。结果两组牵张间隙均充满新生骨痂,在固定期1、14天实验组比对照组新生骨痂更平整连续,在固定期28天两组未见明显差异;石蜡切片HE染色显示实验组较对照组有更多新骨形成,在固定期1、14及28天时,牵张间隙实验组的骨量百分比均高于对照组(P<0.05)。结论失交感神经支配可增强下颌骨牵张成骨新骨形成,提示交感神经系统在牵张成骨过程中起到一定调控作用。
Objective To investigate the effects of sympathetic innervation loss on new bone formation in rat mandibular distraction osteogenesis.Methods A total of 30 male Sprague-Dawley rats were divided into two groups randomly.Control group（group A,n=15） only underwent right mandibular distraction osteogenesis,and experimental group（group B,n=15） underwent bilateral transection of cervical sympathetic trunk（TCST） and then right mandibular distraction osteogenesis.The rats were sacrificed at consolidation time of 1,14 and 28 days,and then the regenerated callus underwent general observation,HE staining and histomorphometric analysis.A two-sample t test was performed using SPSS 12.0 software package.Results General observation showed that the distraction gap was filled with new formed bone callus in two groups.At the consolidation time of 1 and 14 days,the new bone callus of group A was smoother than group B,but no obvious difference at the consolidation time of 28 days.HE staining indicated more bone formation in group B.Histological analysis showed that both new bone volume and thickness of new trabeculae were significantly higher in group B than those in group A（P〈0.05）.Conclusion The sympathetic nerve resection can improve more new bone trabeculae formation,which suggests that the peripheral nerve plays a role during the new bone formation in the distraction osteogenesis.
出处
《创伤外科杂志》
2011年第1期54-58,共5页
Journal of Traumatic Surgery
基金
国家自然科学基金资助(30901685)
关键词
牵张成骨
交感神经
下颌骨
大鼠
distraction osteogenesis
sympathetic nerve
mandible
rats
