摘要
人肠内细菌菌丛或单菌株及其酶与中药化学成分在37℃、厌氧条件下共温孵,转化产物用溶剂法提取、柱色谱法分离、谱学法鉴定化学结构。根据转化产物与原形药物和/或化合物的结构特点,并结合酶催化反应,推断转化机制。建立人肠内细菌转化中药化学成分的模型和标准操作规程,用于研究和评价中药化学成分在肠内的生物转化。所建立的人肠内细菌生物转化模型,操作简单,对中药化学成分结构具有转化能力。转化方式既可采用生长细胞转化法,亦可采用静态细胞转化法或酶转化法。该法可用于研究口服中药化学成分被肠内细菌的生物转化及其转化机制。
Objective： To establish human intestinal bacteria biotransformation model and the standard operation procedure for studying and assessing intestinal biotransformation of chemical constituents of traditional Chinese medicine（TCM）.Method： The chemical constituent of TCM was incubated together with human intestinal flora or isolated strain,or their secretory enzymes at anaerobic environment and 37 ℃.The biotransformation products were extracted by solvent extraction methods,separated by column chromatographic methods,and identified by spectroscopic analysis.The biotransformation mechanisms would be deduced by comparison of structural characteristics of the biotransformation products and the parent drug and/or compound,as well as the enzyme（s）-catalysed bioreactions.Result： The established biotransformation model of human intestinal bacteria is facile for operation and has the capability of converting the chemical constituent of traditional Chinese medicine.The growing cells transformation method,the resting cells transformation method or enzyme（s）-catalysed transformation method can all be selected as the transformation approach.Conclusion： The established human intestinal bacteria biotransformation model can be used to study the intestinal biotransformation of orally administrated chemical constituents of TCM and their biotransformation mechanism.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2011年第1期19-26,共8页
China Journal of Chinese Materia Medica
基金
国家自然科学基金项目(29972004
20272006
20572007
30672609)
"十一五"国家科技支撑计划项目(2006BAI06A01-02)
北京市科委科技专项(Z0004105040311)
国家"重大新药创制"科技重大专项(2009ZX09502-006
2009ZX09301-010)
教育部优秀青年教师基金项目
国家"九五"科技攻关项目(96-C02-03-06)
北京大学"十五211工程"项目
国家高技术领域研究"863"计划项目(2002AA2Z343C
2004AA2Z3783)
关键词
人肠内细菌
中药
生物转化
human intestinal bacteria
traditional Chinese medicine
biotransformation
