摘要
目的:观察戊四氮(PTZ)致痫大鼠海马神经元caspase-3表达以及中药复方AAP的脑保护作用.方法:144只健康成年雄性Wistar大鼠随机分为对照组(CK组)、模型组(PTZ组)、中药大剂量组(AAPl组)、中药中剂量组(AAPm组)、中药小剂量组(AAPs组)和丙戊酸钠组(VPA组);每组各6只.CK组和PTZ组分别给予生理盐水(4mL/kg.d)灌胃;中药各组分别给予中药复方大、中、小剂量(10.26g/kg、5.13g/kg、2.56g/kg)灌胃,每天1次;VPA组腹腔注射VPA(20mg/kg.d).造模第一天,除CK组外,其余各组大鼠均腹腔注射戊四氮(PTZ)75mg/kg,观察记录大鼠行为学变化;于致痫后12h、2d、5d、7d相应时间点取材,制备脑标本;免疫组化检测caspase-3表达.结果:致痫后,除CK组外,其余各组海马区caspase-3阳性表达增强;7天,与PTZ组相比,AAPl组、AAPm组和AAPs组海马CA3区caspase-3阳性表达减弱(P<0.05).结论:caspase-3参与致痫大鼠海马神经元凋亡过程;AAP能降低caspase-3表达,减少神经元凋亡,有神经保护作用.
objective：To investigate the effect of AAP,a Chinese medicine compound,on the expression of caspase-3 in hippocampus of epileptic rats induced by pentetrazole（PTZ）.Methods：114 health mature male Wistar rats were randomly divided into 6 groups（n=6）： Control group（CK group）,model group（PTZ group）,three AAP groups of high,medium and small doses（AAPl group,AAPm group,AAPs group）,sodium valproate group（VPA group）.Control group and model group by an intragastric administration of distilled water（4mL/kg·d）.Three AAP groups by an intragastric administration at doses of 10.26g/kg,5.13g/kg,2.56g/kg respectively.VPA group by an intraperitoneal injection of sodium valproate（20mg/kg·d）.For fhe first day of establishment of the models,seizure was induced by intraperitoneal injection with Pentylenetetrazol（75mg/kg）in all groups other than the control group.Behavioral observation was conducted with Yuhas 5-grade assessment during the establishment of the models.The rats were sacrificed,the brain tissues were sampled and sliced at 12h,2d,5d and 7d following seizures;the expression of caspase-3 was determined by immunohistochemistry.Results：Compared to CK group,theexpression of caspase-3 positive neurons in CA3 subfields increased in all groups;Compared to model group,the expression of caspase-3 positive neurons in CA3 subfields decreased in all the three AAP groups（P0.05）.Conclusions：caspase-3 may play a role in the neuronal apoptosis in PTZ induced seizures,and AAP can reduce the expression of caspase-3 in hippocampus CA3 region in seizure rats,which indicates that AAP may have protective effect on brain damage induced by seizure.
出处
《山东教育学院学报》
2010年第6期39-42,共4页
Journal of Shandong Education Institute
