摘要
阐明康乐霉素Ｃ（Ｋａｎ）对脾细胞增殖和Ｔ－细胞亚型的作用。方法：氚掺入法或噻唑蓝（ＭＴＴ）比色法测定细胞增殖；用荧光激活细胞分选仪（ＦＡＣＳ）测定细胞亚型；曲利苯蓝排斥法测定细胞存活率．结果： Ｋａｎ ８， ４０， ８０和 ４００ ｎｍｏｌ· Ｌ－１，除抑制丝裂原（Ｃｏｎ Ａ， ＰＨＡ和 ＴＰＡ＋ＩＭ）和同种异型抗原刺激的小鼠脾细胞增殖外；与Ｃｉｃ不同，抑制ＬＰＳ （１０ｍｇ·Ｌ－１）刺激的脾细胞增殖；使Ｌ３Ｔ４＋／Ｌｙｔ２＋ Ｔ－细胞亚型比值倒置； Ｋａｎ于 Ｃｏｎ Ａ（ ５ｍｇ· Ｌ－１）刺激后 ２４ ｈ内加入，仍抑制脾细胞增殖． Ｋａｎ Ｂ－４００ ｎｍｏｌ· Ｌ－１不影响脾细胞存活率。结论：与Ｃｉｃ的作用方式不同，Ｋａｎ抑制Ｔ－和Ｂ－细胞活化的早期和晚期时相，抑制细胞增殖，对Ｔｎ－细胞有选择性。
AIM: To elucidate the suppressive effect of kanglemycin C (Kan) on lymphocyte proliferation and T-lymphocyte subsets. METHODS: Splenocyte proliferation was quantified with [3H]thynddine ([3H]TdR ) Pulsing method or 3-(4,5- dhethylthiazol-2-yi ) -2, 5-diphenyltetrazolium bromide(MTT ) colorimetery. L3T4+ and Lyt2+ T-cell subsets were measured with fluorescence-activated cell sorter (FACS). Splenocyte viability was assessed with trypan blue exclusion. RESULTS: Like ciclosporin (Cic)s, Kan 8, 40, 80, and 400 nmol·L-1 inhibited the proliferation of 20%-80 % incubated mouse splenocytes stimulated by concanavalin A (Con A) 5 mg·L-1, phytohemagglutinin (PHA) 5 mg·L-1, tetradecanoylphorbol acetate (TPA) 10μg·L-1 + ionomycin (IM) 0. 5 mg· L-1, and alloantigen (mixed lymphocyte reaction). Kan had no toxicity to the splenocytes at the treated doses. Suppression by Kan was declined with addition the of Kan after culture onset. Furthermore, the suppressive effect of Kan on splenocyte proliferation stimulated by lipopolysaccharides (LPS) 10 mg· L-1 was similar to that on splenocyte proliferation mediated by Con A. Unlike Cic, Kan reversed the ratio of L3T4+/Lyt2+ T-cell subsets. CONCLUSION: Kan had a suppressive action on proliferation of T- and B-lymphocytes and had a selective effect on helper-inducer T-lymphocyte (Th) subset from Cio. Suppression by Kan was timedependent and not associated with toxicity of Kan.
出处
《中国药理学报》
CSCD
1999年第6期546-550,共5页
Acta Pharmacologica Sinica
关键词
康乐霉素C
B-淋巴细胞
T-淋巴细胞
免疫抑制
kanglemycin C
cyclosporine
concanavalin A
phytohemagglutinins
tetradecanoylphorbol acetate
ionomycin
mixed lymphocyte culture test
B-lymphocytes
T-lymphocytes
T-lymphocyte subsets
