摘要
目的探索ＳｉＯ２刺激ＴＨＰ１细胞（具有肺泡巨噬细胞特性的人血单核细胞株）上清液对中国仓鼠肺成纤维（ＣＨＬ）细胞核仁组成区相关的嗜银蛋白（ＡｇＮＯＲｓ）形成及其增殖的关系。方法通过５个系列浓度（０、５０、１００、２００和５００μｇ／ｍｌ）的ＳｉＯ２刺激ＴＨＰ１细胞培养上清液作用于ＣＨＬ细胞，采用综合改良ＡｇＮＯＲｓ染色技术，观察其ＡｇＮＯＲｓ形成并计数；四唑盐（ＭＴＴ）比色法检测ＣＨＬ细胞的增殖。结果随ＳｉＯ２浓度增大，ＣＨＬ细胞ＡｇＮＯＲｓ颗粒均数、颗粒分散度以及ＭＴＴ分析Ａ５７０ｎｍ值均相应增大，呈良好的剂量反应关系，并和ＳｉＯ２尘的肺泡巨噬细胞（ＰＡＭ）的细胞毒性指数呈高度负相关（ｒ＝－０．９６８，Ｐ＜０．０１）。结论ＳｉＯ２刺激的ＴＨＰ１细胞上清液可以引起肺成纤维细胞ＡｇＮＯＲｓ含量的改变，并与成纤维细胞的增殖和ＳｉＯ２尘ＰＡＭ细胞毒性相一致。
Objective To determine whether
AgNORs was involved in silicainduced pulmonary fibrosis,the relationship between AgNORs
staining and proliferation of lung fibroblast induced by SiO2. Methods An improved AgNORs
silver staining technique was employed to study the AgNORs granule counting,and MTT assay
was used to detect proliferation of Chinese hamster lung fibroblasts(CHL) cells,after induction
by THP1 cells(a monocytelike cell line with properties of the pulmonary alveolar macrophage)
supernatant cultured for 36 hours in five different concentrations of silicon dioxide. Results The
mean number of AgNORs granule,dispersity of granules and A570nm values of MTT assay of
CHL cells increased gradually along with the serial concentrations of silica,and were fitted well
in the doseresponse relationship,meanwhile,correlated negatively well with cytotoxicity index
of silica on pulmonary alveolar macrophage(PAM),r=-0.968,P<0.01. ConclusionThe
silicastimulated THP1 cells supernatant could increase the AgNORs amount in CHL cells,which
is consistent with CHL cells proliferative activity and cytotoxicity of silica on PAM.It is
suggested that in the process of silicotic fibrosis,increasing of AgNORs granule in fibroblast
nuclei may play a significant role.
出处
《中华劳动卫生职业病杂志》
CAS
CSCD
1999年第3期148-150,共3页
Chinese Journal of Industrial Hygiene and Occupational Diseases
基金
国家自然科学基金
