摘要
针对半夏生产过程中软腐病废水普遍和危害严重的现状,从7个半夏主产区的半夏植株上分离到47个内生放线菌,通过平板和组培苗拮抗活性筛选获得两个具有较强活性的菌株07JX3-2和07JX3-7,经形态、培养特征和生理生化实验鉴定,初步确定两个分离物均属于链霉菌属(streptomyces),至于其所在种有待进一步鉴定。两个拮抗菌株的获得为半夏软腐病的生物防治研究和应用奠定了基础。
Pinellia ternata is a traditional Chinese herb medicine that has been used in China for over one thousand years.However,cultivated P.ternata is susceptible to a soft rot disease which may cause major loss of yield.In this study,the endophytic Actinomycete are isolated from the plants of P.ternata and identified.Altogether,47 candidate Actinomycete are isolated from surface-sterilized plants of P.ternata.In petri dish tests,2 isolates（07JX3-2 and 07JX3-7） inhibits the growth of strain SXR1.The two isolates significantly reduce the disease incidence on tissue culture seedlings.They are phenotypically identified as Streptomyces.This is the first report on the efficacy of endophytic Actinomycete against bacterial pathogens on P.ternata,which may provide a more environmental and economical alternative to the control of soil-borne disease on P.ternata.
出处
《浙江理工大学学报（自然科学版）》
2011年第1期92-95,共4页
Journal of Zhejiang Sci-Tech University(Natural Sciences)
基金
国家863科技计划项目(2006AA10Z428)
关键词
半夏
内生放线菌
拮抗
分离
鉴定
Pinellia ternata
endophytic actinomycete
antagonism
isolation
characterization
