摘要
目的：建立测定血浆中罗红霉素浓度的ＨＰＬＣＥＣ检测法，并与微生物法的测定结果相比较，评价两种方法的相关性。方法：以克拉霉素为内标，Ｃ１８反相色谱柱，流动相为甲醇乙腈０．０６ｍｏｌ·Ｌ－１磷酸二氢钠０．００３ｍｏｌ·Ｌ－１ＥＤＴＡ（０．１１∶０．３９∶０．４９∶０．０１，ｐＨ７．０），安培型电化学检测器的工作电压＋１．２Ｖ；０．５ｍｌ血浆样品经液液萃取纯化后溶于流动相中，进样２０μｌ。结果：本法的线性范围０．１～１０ｍｇ·Ｌ－１，日内ＲＳＤ＜３．２％，日间ＲＳＤ＜５．５％，方法回收率９９．５％～１０３．３％，最低检测限０．１ｍｇ·Ｌ－１。比较ＨＰＬＣ和微生物法的测定结果，相关性显著（Ｐ＜０．０１）。结论：方法灵敏度高，重现性好，操作简便，适用于罗红霉素的药代动力学研究。
BJECTIVE: To establish a HPLC method for the determination of roxithromycin in human plasma using electrochemical detection,and to compare the results measured by HPLC with that by microbiological assay for assessing the correlation of the two methods. METHODS: Clarithromycin was used as the internal standard.The mobile phase consisted of CH3OHCH3CNNaH2PO4EDTA (0.110.390.490.01) and was adjusted to pH 7.0.The applied potential of the amperometric detection was set at +1.2v and the extracts of samples were chromatographed on a C18 reversedphase column after liquidliquid extraction. RESULTS: The method was linear over the range 0.110mgL-1.The intraday RSD was less than 3.2% and the interday RSD less than 5.5%.Method recovery was 99.5%103.3% and the limit of quantification was 0.1mgL-1.Analytical results for roxithromycin in plasma samples (n=280) between the two methods was found to be correlated lineal (P<0.01). CONCLUSION: The method has good selectivity,sensitivity and reproducibility and has pr
出处
《中国药学杂志》
CAS
CSCD
北大核心
1999年第7期480-483,共4页
Chinese Pharmaceutical Journal
基金
国家自然科学基金
关键词
罗红霉素
血药浓度
HPLC-EC
roxithromycin,electrochemical detection,microbiological assay,correlationoved to be suitable for pharmacokinetic studies of roxithromycin in human.
