2-BFI对EAE小鼠iNOS和COX-2 mRNA表达的影响被引量：11

Intervention effects of 2-BFI on iNOS and COX-2 mRNA in the cerebrum of mice with experimental autoimmune encephalomyelitis

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摘要目的探讨咪唑啉2受体(I2R)高选择性高亲和力配体2-BFI对实验性自身免疫性脑脊髓炎(EAE)小鼠诱导型一氧化氮合酶(iNOS)和环氧化酶-2(COX-2)mRNA表达的影响。方法使用髓鞘少突胶质细胞糖蛋白(MOG35-55)抗原诱导EAE小鼠模型。采用临床症状评分、病理学检查和反转录-聚合酶链反应(RT-PCR)观察完全福氏佐剂(CFA)对照组、EAE组和2-BFI干预组小鼠行为学、中枢炎性细胞浸润及iNOS和COX-2mRNA表达变化。结果 2-BFI干预组较EAE组临床症状明显减轻(P<0.01),中枢炎性细胞浸润显著减少(P<0.01),iNOS和COX-2的mRNA表达水平降低(P<0.05,P<0.01)。结论 I2R高选择性配体2-BFI对EAE小鼠具有一定保护作用,其作用机制可能与降低iNOS和COX-2 mRNA表达有关。 Objective To investigate the effect of 2- （2-benzofuranyl） -2-imidazoline （2-BFI）, which selectively binds to imidazoline 2 receptor （I2R） with high affinity, on experimental autoimmune encephalomyelitis （EAE） mice and the underlying mechanism. Methods Female C57BL/6 mice were randomly divided into three groups： complete freund ＇s adjuvant （CFA） control group, EAE group and 2-BFI treatment group. EAE was induced with myelin oligodendroglia glycoprotein （MOG 35-55）. The behavioral changes and the daffy clinical scores were recorded. The damage and the infiltration of inflammatory cells in central nervous system （CNS） were evaluated with HE stain. Expressions of iNOS and COX-2 mRNA in CNS were quantified by RT-PCR. Results Compared with EAE group, the severity of paralysis （P〈0.01） and the infiltration of inflammatory cells in CNS （P〈 0.01） were improved significantly in 2-BFI treated mice. RT-PCR analysis showed that the expressions of iNOS （P〈0.05） and COX-2 （P〈0. 01） mRNA decreased significantly in the CNS in 2-BFI treated mice compared with EAE group. Conclusions 2-BFI had protective effects on EAE, which may be due to the down-regulations of iNOS and COX-2 mRNA.

作者林福虹郑荣远王沛王赵伟

机构地区温州医学院附属第一医院神经内科

出处《中国神经免疫学和神经病学杂志》 CAS 2011年第1期27-31,共5页 Chinese Journal of Neuroimmunology and Neurology

基金国家自然科学基金资助项目(81070960) 浙江省自然科学基金资助项目(Y2074941)

关键词 2-BFI 实验性自身免疫性脑脊髓炎诱导型一氧化氮合酶环氧化酶-2 2- （2-benzofuranyl） -2-imidazoline experimental autoimmune encephalomyelitis inducible nitric oxide synthase cyclooxygenases-2

分类号 R744.5 [医药卫生—神经病学与精神病学]

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