摘要
目的建立测定复方斑蝥胶囊中野黄芩苷含量的高效液相色谱法。方法采用C18色谱柱(250 mm×4.6 mm,5μm),以甲醇-0.3%磷酸溶液(40∶60)为流动相,检测波长为335 nm。结果野黄芩苷进样量在0.122 76～1.227 6μg范围内与峰面积线性关系良好,加样回收率为98.61%,RSD为0.49%(n=6)。结论高效液相色谱法快速、准确,可用于复方斑蝥胶囊的质量控制。
Objective To determine the content of scutellarin in Compound Banmao Capsules.Methods The HPLC method was adopted and the C18 column（250 mm×4.6 mm,5 μm） was used.The mobile phase was methanol-0.3%phosphoric acid（40 ∶60）.The detection wavelength was at 335 nm.Results The calibration curve of scutellarin was linear in the range of 0.122 76-1.227 6 μg（r=0.999 9）,the average recovery rate was 98.61%,RSD was 0.49%（n=6）.Conclusion The method is quick and accurate,which can be used for the quality control of Compound Banmao Capsules.
出处
《中国药业》
CAS
2011年第1期23-24,共2页
China Pharmaceuticals
