摘要
目的：观察兔关节软骨细胞体外培养形成基质能力。方法：通过Ⅱ型胶原酶消化法获得大量高活性兔关节软骨细胞，倒置显微镜及透射电镜下观察细胞形态及超微结构，用含１５％胎牛血清的ＤＭＥＭ培养液连续培养，观察软骨基质的形成情况。结果：体外培养的软骨细胞为多角形，透射电镜下见胞浆内有丰富的粗面内质网系统及线粒体，胞膜下及胞浆中有较多分泌泡；连续培养１０天时，培养瓶底部出现肉眼可见的白色结节，蕃红－Ｏ染色显示白色结节含大量氨基葡聚糖阳性物质，２０天时形成火山口样结构，也被蕃红－Ｏ染成深红色。结论：软骨细胞在体外也具有很强的基质形成能力并能形成软骨样组织，因而可以用于进行软骨体外再造。
Objective: To observe the ability of matrix synthesis of rabbit articular chondrocytes cultured in vitro. Methods: Rabbit articular chondrocytes were acquired by digestion of 0.l% type Ⅱ collagenase and cultured in vitro, then cell morphology and ultrastructure were observed with phase contrast microscope and scanning electronic microscope. With continuous culturing of chondrocytes in DMEM medium containing 15% FBS, synthesis of cartilage matrix was observed by using microscope and safranine O staining. Results: The cultured chondrocytes were polygonal cells. There were many rough endoplasmic reticula and mitochondria in cytoplasm, and a lot of secretory vesicles under cell membrane and in the cytoplasm. When cultured for 10 days, some small and white nodules were formed on the bottom of the culture dished, and volcanic mouth like structures were formed when cultured for 20 days. Both these nodules and structures contained GAG positive substances were demonstrated by safranine O staining. Conclusion: Chondrocytes can produce matrix and cartilage like tissue in vitro, so it is feasible to produce cartilage by culturing chondrocytes in vitro.
出处
《华西口腔医学杂志》
CAS
CSCD
北大核心
1999年第2期110-112,I005,共4页
West China Journal of Stomatology
