摘要
用辣根过氧化物酶标记的抗小鼠肝炎病毒(MHV)单克隆抗体(McAb),建立了ELISA 双抗体夹心法,并与 ELISA 间接法检测小鼠血清中的 MHV 抗体进行了比较。发现两种方法在检出率、特异性、稳定性方面并无明显差异,但由于间接法操作更为简便,因此认为,检测血清中 MHV 抗体采用间接法较好。
Double antibody sandwich ELISA is established by using McAbs against MHV with horseradish peroxidase,this method is compared with indirect ELISA in detecting antibody against MHV in serum of mouse. There is no clear difference in screening rate,stability,specificity.Because it is more conventional in routing diagnosis,indirect ELISA is better than double antibody sandwich assay in detecting antibody against MHV.
