摘要
目的:研究大豆异黄酮对血管紧张素Ⅱ(AngⅡ)诱导的血管平滑肌细胞(VSMC)增殖的影响并探讨其作用机制。方法:分离大鼠胸主动脉,组织贴块法培养VSMC,以AngⅡ为诱导剂,建立VSMC细胞增殖模型。应用MTT法,CellTiter-GloR As-say法检测细胞增殖,观察不同浓度的大豆异黄酮及亚硝基-精氨酸甲酯(L-NAME)对AngⅡ诱导的VSMC增殖的影响。硝酸还原酶法检测细胞上清液中一氧化氮(NO)含量;化学比色法测定一氧化氮合酶(NOS),诱导型一氧化氮合酶(iNOS)水平;半定量RT-PCR检测VSMC iNOS mRNA的表达。结果:大豆异黄酮能剂量依赖性的抑制VSMC增殖,该作用可被L-NAME部分抵消;大豆异黄酮能升高NOS、iNOS和NO水平,增加iNOS mRNA的表达。结论:大豆异黄酮可呈剂量依赖性的抑制AngⅡ诱导的VSMC增殖,该作用可能通过上调iNOS基因表达、升高NO水平实现的。
Objective： To investigate the effects of soy isoflavone on the proliferation of cultured rat vascular smooth muscle cell（VSMC） induced by angiotensin Ⅱ.Methods：VSMCs were primary cultured by tissue sticking method.Cell proliferation model was established by stimulation with AngⅡ.Cell proliferation was measured by MTT assay and CellTiter-Glo assay to observe the effects of various concentrations of soy isoflavone and NG-nitro-L-arginine methyl ester（L-NAME,100 μmol/L） on VSMC proliferation induced by AngⅡ.Nitric oxide（NO） level was measured by Griess reagent.Nitric oxide synthase（NOS） and inducible nitric oxide synthase（iNOS） levels were detected by chemical colorimetric method.mRNA expression of iNOS was measured by reverse transcription polymerase chain reaction（RT-PCR）.Results： Soy isoflavone ranging from 0.1～1.0 μmol/L inhibited cell proliferation in a dose-dependent manner.The inhibitory effects were partly blocked by 100 μmol/L of L-NAME.Soy isoflavone markedly increased NO,NOS and iNOS levels,and increased iNOS mRNA expression in VSMC.Conclusion： Soy isoflavone could inhibit VSMCs proliferation induced by AngⅡ.The increase of NO secretion may participate in this progress.
出处
《南京医科大学学报（自然科学版）》
CAS
CSCD
北大核心
2010年第12期1713-1717,共5页
Journal of Nanjing Medical University(Natural Sciences)
基金
南京医科大学科技发展基金面上项目资助(08NMUM067)
