摘要
为建立广东化州市特有药材化橘红的高效液相色谱(HPLC)指纹图谱,采用色谱条件为:Nucleosil C18反相柱(150mm×4.6mm,3μm),DAD检测器,流动相为水-体积分数50%甲醇溶液+体积分数15%～50%乙腈溶液,三元梯度洗脱,流速为0.3～1mL/min,柱温35℃,分离波长315nm。结果表明:11批次的化橘红提取物色谱图共有峰9个;经"中药指纹图谱鉴别分析系统"统计分析,共有峰相对保留时间的相对标准偏差在0.3%以下,相似度均在0.965以上。本法符合指纹图谱的要求,可建立共有模式,作为科学评价化橘红产品质量的检测方法。
In order to establish the fingerprint of HPLC analysis for tomentose pummelo （Exocarpium citri Grandis） peel, reversed-phase HPLC using Nucleosil C18 column （150 mm × 4.6 mm, 3μ m） as the stationary phase, H20-CH3OH-CH3CN as the mobile phase, triple gradient elution at the flow rate of 0.3 - 1 mL/min, DAD as the detector, column temperature at 35℃ and detection wavelength at 315 nm was used to determine the extract from tomentose pummelo peal. Results showed that 9 common peaks in HPLC chromatogram were observed in 11 samples. Through calculating by fingerprint analysis system of Chinese medicine, relative standard deviations （RSD） for relative retention time of common peaks were less than 0.3% and their similarity was more than 0.965. Therefore, a chromatographic fingerprint was established to provide scientific quality evaluation for tomentose pummelo peel.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2010年第22期318-321,共4页
Food Science
基金
广东省科技厅农业攻关项目(2006B20201045)
关键词
HPLC
化橘红
香豆素
指纹图谱
HPLC
tomentose pummelo peel
coumarin
fingerprint
