摘要
目的:探索螺旋藻(Spirulina)碱性蛋白酶酶解肽对家兔腹腔黏膜系统的作用。方法:采用盐析法从螺旋藻中提取可溶性螺旋藻蛋白,以碱性蛋白酶水解得到螺旋藻蛋白水解物溶液。将所得水解物以3种方式(口服、腹腔注射、耳静脉注射)对家兔给药,分别在给药3、2、2h后,用ELISA试剂盒测定血清中IL-1β、IL-6、IL-12、IFN-γ、TNF-α、IL-1Ra、IL-4、IL-10、TGF-β细胞因子的质量浓度。结果:3个实验组与正常生理盐水组相比,IL-12质量浓度均下降,IFN-γ、IL-10质量浓度均升高;在3种给药方式中,腹腔注射效果最显著,其他两组细胞因子水平均没有显著性变化。腹腔注射组中炎症细胞因子IL-1β、IL-6、IL-12、TNF-α的质量浓度均显著性降低,抗炎细胞因子IL-1Ra和IL-4显著性升高,说明该螺旋藻碱性蛋白酶酶解肽对降低免疫活性起到了一定作用。结论:肠黏膜系统对螺旋藻蛋白酶解肽信号传递主要是通过肠道上皮细胞、内皮细胞及单核/巨噬细胞、淋巴细胞之间的信号网络抑制NF-κB和PKC/p38/MAPK途径来降低免疫的。这与植物非营养物质能够通过抑制转录因子NF-κB活性抑制炎症信号级联的作用机制相一致,说明短肽类成分也可以进入体内对机体的慢性疾病甚至癌症起到一定的治疗作用。
Objective：To explore the effects of peptides derived from Spirulina protein hydrolyzed by alkali protease on mucosal immune system in rabbits.Methods：Protein was extracted from Spirulina by salting out method,and then hydrolyzed with alkaline protease.The rabbits were treated with the hydrolysate by intragastric administration,intraperitoneal injection and ear vein injection,respectively.At 3,2 and 2 h after administration,ELISA kit was used to determine the concentration changes of IL-1β,IL-6,IL-12,IFN-γ,TNF-α,IL-1Ra,IL-4,IL-10 and TGF-β in rabbit serum.Results：Compared with the control group treated with physiological saline,all the three administration route groups showed a notable decrease in the concentration of IL-12 but an increase in both the concentrations of IFN-γ and IL-10.However,on the basis of the comparison among the three administration routes,intraperitoneal injection revealed the most remarkable change in cytokines;in contrast,the two others had no obvious change.In the intraperitoneal injection group,the concentrations of IL-1 β,IL-6,IL-12 and TNF-α revealed a significant decrease,while those of IL-1Ra and IL-4 exhibited a dramatic increase,which suggests that the peptides derived from Spirulina protein hydrolyzed by alkali protease have strong immune activity.Conclusion：The immune signaling transduction of Spirulina peptides in mucosal immune system is completed by inhibiting NF-κB and PKC/p38/MAPK pathways in mucosal epithelium,endothelial cell,monocytes/macrophages and lymphocytes,which is similar to the phytochemical mechanism for inhibiting NF-κB and inflammatory reaction.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2010年第21期378-383,共6页
Food Science
