摘要
为探讨蜂胶醇提物对人肝癌细胞Hep G2增殖和凋亡的影响,采用MTT法检测蜂胶醇提物对Hep G2细胞增殖的抑制作用,用荧光倒置显微镜和流式细胞仪分析蜂胶醇提物对Hep G2细胞凋亡的影响。结果表明:12.5～200μg/mL质量浓度的蜂胶醇提物作用24、48h后,均能不同程度地抑制Hep G2细胞的增殖,呈明显的时间、剂量依赖关系,半数抑制质量浓度(IC50)分别是115、78μg/mL。作用8h后,Hep G2细胞出现不同程度的凋亡,凋亡率由6.36%上升到21.9%,呈现出剂量依赖关系。故蜂胶醇提物可显著抑制人肝癌细胞Hep G2的生长,诱导其凋亡。
In order to investigate the effect of propolis on cell proliferation and apoptosis,the ethanol extract of propolis was used to treat human liver carcinoma Hep G2 cells.The inhibitory rate of ethanol extract from propolis on the proliferation of human liver carcinoma Hep G2 cells was determined by MTT assay and the apoptosis of human liver carcinoma Hep G2 cells was examined by using inverted fluorescence microscope and flow cytometry analysis.Results indicated that 12.5-200 μg/mL ethanol extract of propolis exhibited an obvious inhibition effect on the proliferation of Hep G2 cells after 24 h and 48 h treatment in a time-and dose-dependent manner.The apoptosis of Hep G2 cells was obviously observed after treatment and apoptosis rate was in the range of 6.36% to 21.9% in a dose-dependent manner.These results confirm that propolis can significantly inhibit the proliferation and induce apoptosis of Hep G2 cells.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2010年第21期344-348,共5页
Food Science
基金
国家现代农业(蜂)产业技术体系资助项目(NYCYTX-43)
国家公益性行业(农业)科研专项经费项目(nyhyzx07-041)
关键词
蜂胶
HEP
G2细胞
增殖
凋亡
propolis
Hep G2 cell
proliferation
apoptosis
