摘要
目的:应用最新生化处理技术研制了一种以动物组织为原料的去抗原生物型人工韧带,检测其结构、力学性能及组织相容性,探讨应用去抗原生物型人工韧带重建膝关节叉韧带的可行性。方法:对动物肌腱/韧带组织进行环氧交联固定、多方位去抗原、力学增强改性以及表面活性修饰处理制得生物型人工韧带,应用组织学检查、扫描电镜、细胞培养及力学测试等多种方法分析生物型人工韧带的组织结构、生物力学特性和体内/体外组织相容性。结果:生物型人工韧带的外观与正常肌腱外观相似。组织学检查显示韧带为无细胞的胶原纤维。电镜检查显示韧带为走向一致的发状纤维样物紧密平行排列。异种骨髓基质细胞于韧带表面培养3周,可见细胞黏附并可见细胞分泌的基质沉积于细胞周围,韧带内部未见细胞。人工韧带平均直径约5 mm。在速度100 mm/m in条件下进行力学测试,平均最大拉力为927.19 N,抗拉强度47.22 N/mm2,与正常山羊的前交叉韧带(ACL)相应参数相近。人工韧带置换羊ACL术后12周,韧带表面可见滑膜组织覆盖并有小血管长入,术后52周人工韧带材料被新生的韧带替代,近关节面骨隧道内的人工韧带材料纤维周边可见韧带-纤维软骨-钙化软骨-骨的直接连接模式。结论:应用新的生化处理技术研制的生物型人工韧带有效地去除了猪肌腱的异种蛋白的免疫原性,具有良好的生物力学特性和组织相容性,作为膝关节叉韧带重建的韧带替换材料具有良好的应用前景。
AIM：To detect the structure,mechanical properties and biocompatibility of an artificial biological ligament（ABL）,and to explore the feasibility of reconstructing the anterior cruciate ligament（ACL） with ABL.METHODS： By means of treating porcine tendon with epoxy cross-linking fixation,diversified antigen minimization process,mechanic enhancement modification and surface activating process,the ABL samples were fabricated in a goat implantation model.The in vivo and in vitro performances of ABL were analyzed by histological examination,scanning electron microscopy,cell culture and mechanical tests before and after animal implantation.RESULTS： The appearance of ABL was similar to that of the normal human ligament.Histological examination showed that the ABL was cell-free collagen fibers.Electron microscope examination revealed that the ABL was composed of hair-looking and fiber-like objects running uniformly in a certain direction and closely parallel-arranged.After cultured with xenogenic marrow matrix cells on the surface of ABL for 3 weeks,the cells adhered to the ABL and the matrix apposition around the cells was observed.No cells were found inside the ABL.The average diameter of ABL was 5 mm and the mechanical test at a speed of 100 mm/min showed the average tensile limit of 927.19 N and the tension-resistant strength of 47.22 N/mm,which were closed to the corresponding parameters of normal goat＇s ACL.Twelve weeks after the replacement of goat ACL with ABL,the synovial membrane was observed to cover with the ingrowths of small blood vessels on the surface of the implant.Fifty weeks after the replacement,the ABL material was partially replaced by regenerated host ligament-like tissue.The direct connection mode of ligament-fibro cartilage-calcified cartilage-bone was established around the ABL material fibers in the bone tunnel close to the joint surface.CONCLUSION： ABL has acceptable biomechanical properties,superior biocompatibility and promising prospect for clinical applications.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2010年第12期2394-2399,共6页
Chinese Journal of Pathophysiology
基金
广东省科技计划基金资助项目(No.2006B60501020)
关键词
人工生物型韧带
环氧交联固定
多方位除抗原
表面活性修饰
生物力学特性
生物相容性材料
Artificial biological ligament
Epoxy cross-linking fixation
Diversified minimizations of antigen
Surface activating process
Biomechanical properties
Biocompatible materials
