摘要
本研究旨在探索血管紧张素Ⅱ受体1型基因at1在髓系白血病患者样本中的at1 mRNA表达水平及其与白血病细胞比例、外周血Hb、WBC、Plt计数之间的相关性。收集51例患者骨髓或外周血样本,其中43例为髓系白血病标本(AML组36例、CML组7例);8例非恶性血液性疾病患者骨髓作为对照组。采用RT-PCR方法测定at1 mRNA表达水平,用2-△△CT法对at1mRNA表达水平进行相对定量分析并与对照组进行比较。结果表明:AML组、CML组和对照组at1 mRNA均值相对表达水平分别为0.038±0.076,0.033±0.039,0.281±0.366,髓系白血病患者at1 mRNA的表达与对照组相比明显减低,在AML样本中at1基因mRNA表达与白血病细胞比例呈负相关,与外周血Hb水平呈正相关。结论:at1基因在白血病细胞增殖中可能没有重要作用,而与红系造血有一定关系。
This study was aimed to explore the expression level of angiotensin-Ⅱ type 1 receptor（AT1） mRNA in bone marrow of myeloid leukemic patients, and its correlation with the proportion of leukemical cells in samples and Hb, WBC, Plt counting in peripheral blood. 51 samples,including 36 AML, 7 CML, and 8 samples of non-malignant hematological diseases as control group were collected. The expression of at1 mRNA was detected by real time-PCR; the expression levels of at1 gene in AML and CML groups were relatively quantitatively analyzed by using 2-△△CT and were compared with control group. The results showed that the expression levels of at1 mRNA in AML, CML and control groups were 0.038±0.076,0.033±0.039,0.281±0.366, respectively. at1 gene expression in the myeloid leukemic group was significantly lower than that in the control group. The expression level of at1 mRNA in AML was negatively corralated with the proportion of leukemia cells and positively with Hb level in peripheral blood. It is concluded that at1 gene may play a minor role in leukaemogenesis, however, may promote erythropoiesis.
出处
《中国实验血液学杂志》
CAS
CSCD
2010年第6期1418-1421,共4页
Journal of Experimental Hematology
