摘要
本实验通过稳态荧光法研究了水溶液体系中盐酸小檗碱(HB)与乙烯吡咯烷酮(VP)和β-环糊精(β-CD)的相互作用。定量地研究了荧光光谱的变化,结果表明,β-环糊精的加入使盐酸小檗碱的荧光显著增强,而乙烯吡咯烷酮则导致其荧光减弱,并证实形成了1:1包合物,还计算了其在不同温度下的包络形成常数K_f及其热力学函数。
The interactions of hydrochloridum berberini with 1-vinyl-2-pyrrohdone and β-cyclodextrin in aqueous solution were studied by spectrofluorimetric method. The spectral changes were quantitatively investigated. Results showed that the com-plexation with β-cyclodextrin resulted in an enhancement of the fluorescence of hydrochloridum berberini whereas 1-vinyl-2-pyrroli-done caused a quenching of the fluorescence. It had been supposed 1:1 complex formation. Specifically, formation constants and the thermodynamic parameters at different temperatures were calculated.
出处
《中药材》
CAS
CSCD
1999年第6期307-309,共3页
Journal of Chinese Medicinal Materials
关键词
盐酸小檗碱
乙烯吡咯烷酮
Β-环糊精
稳态荧光法
Hydrochloridum berberini, β-Cyclodextrin
1-Vinyl-2-pyrrolidone
Spectroflurimetry
Formation constant
