摘要
目的研究脂蛋白（ａ）是否能刺激肝细胞纤溶酶原激活物抑制物１（ＰＡＩ１）的分泌。方法将不同浓度的脂蛋白（ａ）加到培养的ＨｅｐＧ２细胞，应用酶联免疫吸附测定（ＥＬＩＳＡ）技术测定细胞上清液中的ＰＡＩ１抗原，采用逆转录多聚酶链式反应（ＲＴＰＣＲ）观测ＨｅｐＧ２细胞中ＰＡＩ１ｍＲＮＡ的表达。结果脂蛋白（ａ）能刺激ＨｅｐＧ２细胞ＰＡＩ１抗原的分泌并具剂量效应关系，同时ＰＡＩ１ｍＲＮＡ表达增加。结论脂蛋白（ａ）能刺激肝细胞ＰＡＩ１基因表达，脂蛋白（ａ）的这种作用可能参与了其致动脉粥样硬化的病理过程。
Objective To examine whether lipoprotein () can stimulate the secretion of
plasminogen activator inhibitor type 1 (PAI1) in cultured hepatic cell Methods Different
concentrations of lipoprotein () were added to cultured HepG2 cells PAI1 antigen in the
supernatant was measured by ELISA while PAI 1 mRNA expression assessed through
reverse transcriptionpolymerase chain reaction (RTPCR) technique Results Lipoprotein
stimulated the secretion of PAI1 antigen by HepG2 cells with a doseeffect relation and
enhanced PAI1 mRNA expression simultaneously Conclusion Lipoprotein () can stimulate PAI
1 gene expression in cultured hepatic cells and this effect may be involved in atherogenesis
lipoprotein
出处
《中华心血管病杂志》
CAS
CSCD
北大核心
1999年第3期231-233,共3页
Chinese Journal of Cardiology
