摘要
目的:通过对二仙汤及其组方中药对幼年大鼠子宫作用的定量分析,探讨其对子宫功能的影响机制。方法:幼年SD大鼠54只,分为正常对照、己烯雌酚、二仙汤、淫羊藿、仙茅、巴戟天、当归、黄柏和知母9组,各组给药6 d后麻醉大鼠,取血清测量雌二醇(E2)的含量,分离摘取双侧子宫,称重后常规石蜡包埋切片,行HE染色和免疫组织化学染色。结果:己烯雌酚组、二仙汤组、淫羊藿组和仙茅组子宫系数与正常组对比增加明显,有统计学意义(P<0.05)。仅己烯雌酚组E2增高。HE染色发现己烯雌酚组子宫内膜厚度和上皮细胞高度明显增加(P<0.01)。免疫组织化学染色发现己烯雌酚可明显下调雌激素受体α(ERα)和雌激素受体β(ERβ)在大鼠子宫中的表达,而二仙汤及方中君药淫羊藿和仙茅则上调ERα和ERβ的表达(P<0.01)。结论:二仙汤及其方中温肾药(淫羊藿、仙茅)通过调节雌激素受体ERα和ERβ在子宫中的表达而发挥植物雌激素的功能。
Objective：To explore the possible functional mechanism for effect of Er-xian Decoction on uterus through quantity analysis of effect of Er-xian Decoction and its compositions on uterus in immature rat.Method：Fifty-four immature female SD rats were randomly divided into nine groups： normal control group（A）,diethylstilbestrol（DES） group（B）,Er-xian Decoction group（C）,Herba Epimedii group（D）,Rhizoma Curculiginis group（E）,Radix Morindae Officinalis group（F）,Radix Angelicae Sinensis group（G）,Cortex Phellodendri group（ H）, Rhizoma Anemarrhenae group （I）. After the rats were administrated for six days, serum were got to measure Estradiol（E2）. After bilateral uterus was removed and weighed, paraffin sections, HE staining and immunohistochemical staining were made. Result：The uterus coefficient was increased significantly in Group B, C, D and E （P 〈 0.05）. The level of E2 in Group B only was increased. It was found diethylstilbestrol （DES） could induce increasing in the height of endometrium epidemic cells and the height of endometrium sreoma cells. Immunohistochemical staining showed that DES caused a decrease in the expression of ERα and ERβ in the uterus of immature rats, but Er-xian Decoction and its kidney-warming composition （Herba Epimedii, Rhizoma Curculiginis） could up-regulate the ERα and ERβ expression. Conclusion： Er-xian Decoction and its kidney- warming composition （Herba Epimedii, Rhizoma Curculiginis） could act as the phytoestrogen by upregulating the expression of ERα and ERβ in the uterus of immature rats.
出处
《中国实验方剂学杂志》
CAS
北大核心
2010年第18期118-121,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家自然科学基金项目(30772849)
高等学校创新引智计划项目(B07007)
北京中医药大学科研基金(0100013)
关键词
二仙汤
子宫
雌激素受体
大鼠
Er-xian Decoction
uterus
estrogen receptor
rat
