摘要
目的:从枫杨树皮中寻找抗菌活性成分,揭示其抗菌作用的物质基础及发现新抗菌素。方法:生物活性跟踪法;体外抗菌为检测指标;萃取、沉淀、硅胶吸附柱色谱、Sephadex LH-20凝胶柱色谱的为分离手段;EI-MS,1H-NMR,13 C-NMR和DEPT为结构鉴定手段。结果:筛选到一个对卡拉双球菌、白色念球菌、小麦赤霉、沙门氏菌、枯草芽孢杆菌、棉花球菌、放线菌等有显著拮抗作用的单体,结构鉴定为5-羟基-2-乙氧基-1,4-萘醌。结论:该活性单体与已知抗菌物质胡桃醌仅有一个2-乙氧基的区别,揭示此类结构母核具有抗菌开发价值。本研究明确了枫杨抗菌作用的物质基础,也为枫杨药材质量控制和研发枫杨活性部位抗菌外用药奠定了基础。
The purpose was to screen antibacterial constituent from the barks of Pterocarya stenoptera,which was a Chinese traditional drug often used to treat bacterial and fungous skin diseases for hundreds of years.Through extraction,precipitation,silica gel column adsorption chromatography,Sephadex LH-20 gel chromatogra-phy using a bioassay-guided fractionation technique（An antibacterial experiment in vitro was used as the screening technique）,a red needlelike liposoluble compound with excellent antibacterial activity to Diplococcus cata, Strepto-coccus albus, Gibberella saubinetii, Salmonella sp. , Bacillus subtilis, and so on, was isolated from the barks of P. stenoptera. The compound was identified as 2-ethoxy-5-hydroxynaphthalene-1,4-dione （ C12H10O4, M = 218） by EI- MS, ^1H-NMR, ^13C-NMR and DEPT. The structure of another antibacterial compound, juglone （Azadbakht M, 2005） , is very similar to 2-ethoxy-5-hydroxynaphthalene-1,4-dione except for a 2-ethoxy, which suggest that this kind of chemical structure may has potential value for development and application of new antibacterial compound. The results in this paper had not only revealed the antibacterial activity substance of of P. stenoptera, but also laid the foundation for the study of quality standard of P. stenoptera and antibacterial active section for externally used.
出处
《中国实验方剂学杂志》
CAS
北大核心
2010年第18期82-84,共3页
Chinese Journal of Experimental Traditional Medical Formulae
基金
广西壮族自治区教育厅面上项目(200810MS039)
关键词
枫杨
抗菌活性
生物活性跟踪法
分离纯化
NMR
EI—MS
萘醌
Pterocarya stenoptera
antibacterial activity
bioassay-guided fractionation technique
isolation and purification
NMR
EI-MS
naphthoquinone
