摘要
microRNA(miRNA)是一类广泛存在于真核生物中,不编码蛋白质的短序列RNA,它广泛参与真核生物的生长发育、新陈代谢和应激反应等生命活动。但绝大多数miRNA的生物学功能还不清楚,通过灵敏的定量检测方法,了解miRNA在不同组织部位的时空表达,是探究其功能的重要环节。现着重介绍了2类7种基于PCR技术的miRNA定量检测方法的基本原理和实验流程,并分析了这些定量检测技术间的异同和适用范围。
MicroRNA（miRNA）is a class of-21nt non-coding RNAs that regulate mRNAs at the post-transcriptional level,and widely play roles in eukaryotic growth and development,metabolism and stress responses.However,the biological functions of most miRNAs remain unclear.Investigating miRNAs＇ spatial and temporal expression pattern through sensitively quantitative detection methods in different tissues,which is an important job to explore their functions.Therefore,focused on the basic principles and laboratory procedures of two classes and seven quantitative detection methods which based on PCR technology,and the differences and applications among them were analyzed.
出处
《中国生物工程杂志》
CAS
CSCD
北大核心
2010年第11期88-93,共6页
China Biotechnology
基金
国家"973"计划资助项目(2010CB126601)
