摘要
目的:探讨出生后小鼠小脑颗粒细胞在体电转染外源性基因的方法。方法:用在体电转仪对出生后小鼠小脑表面电转染绿色荧光蛋白质粒,在电转染后的不同时间点观察小脑绿色荧光蛋白基因的表达及绿色荧光蛋白阳性细胞的迁移和分化。结果:电转染后24h即可在小脑皮质观察到绿色荧光,并可持续到电转后10d以上。出生后15d内绿色荧光蛋白阳性细胞陆续向小脑内颗粒细胞层迁移并分化为小脑颗粒细胞。结论:出生后在体电转染技术能有效地将外源性基因导入到小脑颗粒细胞中表达。
To explore the method for in vivo electroporation on mouse cerebellum postnatally. Methods： The plasmid of CAG-EGFP was transfected to cerebellar cortex surface at the fifth day after birth by in vivo electroporators. The EGFP gene expression, the migration and differentiation of EGFP positive cells at different time points after electroporation were observed. Results： The EGFP fluorescence could be found in cerebellar cortex as early as 24 hours after electroporation, and it could last for at least ten days. During the early fifteen days after birth EGFP postitive cells migrated to internal granular cell layer, and differentiated to granular cells in succession. Conclusion： Exogenous gene can be transfected postnatally into cerebellar granular cells by in vivo electroporation, providing a reliable method for exogenous regulating gene expression and tracing cell migration.
出处
《中国临床神经科学》
2010年第6期573-577,共5页
Chinese Journal of Clinical Neurosciences
基金
国家杰出青年基金项目资助(编号:30725019)
关键词
在体电转染
小脑
基因
小鼠
in vivo electroporation
cerebellum
gene
mouse
