摘要
目的:建立一种快速、有效、陈旧血样基因组DNA的提取方法。方法:采用SDS,EDTA,Tris,蛋白酶K作细胞裂解剂裂解细胞和细胞核,用Tris饱和酚和氯仿:异戊醇(24:1)直接提取基因组DNA。结果该方法提取陈旧血样基因组DNA的纯度高。结论:本法简便、有效,适用于批量陈旧血样的处理。
Objective:To esta blish a rapid,effective,old blood genomic DNA extraction method.Methods:SDS,EDTA,Tris,proteinase K cell lysis agent for cell lysis and nuclear,with Tris saturated phenol and chloroform:isoamyl alcohol(24:1) direct extraction of genomic DNA.Results The blood samples of genomic DNA from old high purity.Conclusion:This simple,effective and suitable for mass treatment of old blood samples.
出处
《畜禽业》
2010年第12期35-36,共2页
Livestock and Poultry Industry