摘要
应用机械分离技术获得豚鼠单离初级精母细胞后，进行全细胞式膜片钳记录。结果表明；在标准盐溶液中，当钳制电位－90mV，指令电压（－60～＋140）mV，步阶电压20mV时，可记录到外向离子电流。将标准盐溶液换成含4mmol／L的TEA盐溶液，外向电流显著抑制。冲洗换液后大部分电流恢复，表明此电流为外向K离子流。
By whole-cell patch clamp technique,outward potassium-ion currents in guinea pigprimary spermatocytes were recorded. The experimental results showed that outward currents were induced at a holding potential of -90 mV a cPmmand potential from -60 mV to+140 mV,a step potential of 20 mV in standard saline. while the outward current were decrcased obviously in TEA saline. These results indicate that the outward currents were carriedby k+.
关键词
精母细胞
膜片钳
外向电流
spermatocytes
patch-clamp
outward current
