摘要
探讨基因蛋白ｂｃｌ－２，Ｆａｓ的表达变化与胃癌及癌前病变之间的关系。方法采用免疫组化ＳＰ法，分别检测浅表性胃炎组织２０例，萎缩性胃炎３２例，肠化生３２例，胃癌３６例基因蛋白ｂｅｌ－２，Ｆａｓ的表达变化。结果Ｆａｓ基因蛋白的表达，浅表性胃炎组６５％（１３／２０）较肠化生组２１．８％（７／３２）及胃癌组１９．６％７／３６）明显增高（Ｐ＜０．００５）；ｂｃｌ－２基因蛋白表达，胃癌组织３３．３％（１２／３６），其中高一中分化腺癌阳性表达５６．２５％（９／１６）较低分化腺癌１６．６％（２／１２）及未分化癌１２．５％（１／８）明显增高（Ｐ＜０．０５），浅表性胃炎组无回例表达；胃炎与胃癌组之间Ｆａｓ和ｂｃｌ－２表达呈负相关，两者比较有显著性差异（Ｐ＜０．０５）。结论ｂｃｌ－２基因的异常表达对胃癌及癌前病变的形成发挥一定作用；Ｆａｓ基因的过度表达诱导胃炎发生，而弱表达又为胃粘膜细胞癌变提供了条件；ｂｃｌ－２与Ｆａｓ基因的表达失衡可能与胃癌的发生有关。
Aim Tostudy the relationship between gene-protein bcl-2 Fas expression altelations and gastric cancerPrecancerous lesion.Methods Expressions of gene-protein bcl-2 and Fad were detected in 20 Saperficial gastritis tissues. 32ample gastritis tissues. 32 intestinal metaplasia tissues and 36 gastric cancer lesion tissues respeCtively by immunohis tochemical staining techniqUe (sp method). Resutu Positive Fas expression of superficial gastritis was observed in 65% (13/20),which was significantly higher than intestinal metaplasia lesions 21. 8% (7/32) and gastric cancer lesions 19. 6% (7/36) (P< 0. 005 ); positive bcl-2 expression of gastric cancer was investigated in 33. 3 % (12/36), being higher in the well differentiated 56.25% (9/16) than foe pooly differentiated 16. 6% (2/12) and nodifferentiated gastric cancer 12. 5% (1/8) (P< 0 .05), Supcefficial gastritis w negative with bcl-2 mpression; expressions of hcf-2 and Fas were showed just the oppeitebetween the gastric lesions and gastric cancer lesion tissues (P < 0. 05). COncluSionS Aberrant bcl-2 expression Plays certain role in gastric cancer and precancerous lesion fonnations; Fas gene overexpression induce gastritis happening, but Fad underexpr-ession offer certain condition to gastric catcinogenesis; boalance of bcl-2 and Fas expression may cormlate with gastriccarelnogenesls.
出处
《胃肠病学和肝病学杂志》
CAS
1999年第1期27-29,共3页
Chinese Journal of Gastroenterology and Hepatology
