摘要
对散斑壳属2个近似种,即松针散斑壳(Lophodermium pinastri)和乔松散斑壳(L.pini-excelsae)的外部形态及内部构造进行了比较研究,并绘图示之。同时对此二种多个菌株的rDNA-ITS序列进行了PCR扩增与测定,并与GenBank中已有的相关序列进行同源性分析;利用MEGA邻接法(neighbour-joining method,NJ)构建了系统发育树。结果表明,在形态解剖学方面,L.pinastri除子座基部层、线纹及分生孢子器开口特征外,其余重要特征与L.pini-excelsae具有很大的相似性。L.pinastri ITS序列的G+C含量(51.5%)大于L.pini-excelsae的G+C含量(50.1%)。从系统发育树可以看出,双维松上的L.pinastri和单维松上的L.pini-excelsae形成2个明显独立的分支,寄主对种内遗传多样性也产生较重要影响,产地与种内遗传差异无明显关联。ITS序列分析可以作为区分这2个近似种的一种有效手段。
Macroscopic appearance and internal structure of two related species of Lophodermium pinastri and L. pini-excelsae were described and illustrated. At the same time,rDNA-ITS sequences of some strains of the two species were amplified and determined,and the genetic homology among these sequences and other related sequences in GenBank was analyzed. A phylogenetic tree of these strains was constructed by MEGA using neighbour-joining method. Results show that the internal characteristics of L. pinastri are remarkably similar to those of L. pini-excelsae,except those of stroma basic layer,zone lines and opening of pycnidia. G＋C content of L. pinastri（51.5%） was higher than that of L. pini-excelsae（50.1%）. The phylogenetic tree indicates that L. pinastri on diploxylon pines and L. pini-excelsae on haploxylon pines can be sharply classified into two independent groups,and hosts have a definite impact on intraspecific genetic diversity. Origin is not significantly associated with intraspecific genetic variation. ITS sequence analysis is an effective means to distinguish the two related species.
出处
《东北林业大学学报》
CAS
CSCD
北大核心
2010年第5期101-104,共4页
Journal of Northeast Forestry University
基金
国家自然科学基金项目(30870014
30770006)
教育部高校博士点专项科研基金项目(20070364002)资助
关键词
斑痣盘菌科
形态解剖学
序列分析
亲缘关系
Rhytismataceae
Morphological characteristics
Sequence analysis
Genetic relationships
