摘要
建立了高效液相色谱检测水体中微囊藻毒素(Microcystins,MCs)的方法。选择XAD-2树脂为富集树脂,采用四氯化碳-10%乙醇-10%丙酮连续淋洗,获得较好的杂质去除效果,采用90%甲醇可以将树脂柱上的MCs完全洗脱。HPLC采用V(0.1%TFA):V(甲醇)=42∶58混合溶液为流动相进行等度洗脱,MC-RR和MC-LR取得较好分离效果,回收率均达到90%以上。水样富集2000倍后,方法的检出限为0.05μg/L。本方法操作简便、灵敏度高、检测速度快。
The determination method for microcystins （MCs） in aquatic environment with high-performance liquid chromatography was improved. The results showed that good effects on the rinse of impurity was obtained by using carbon tetrachloride-10% ethanol-10% acetone as the eluate, and all the MCs in XAD-2 resin column could be eluted by 90% methanol-water solution. In the experiment, MC-RR and MC-LR could be totally separated at isocratic elution with mobile phase V（0.1% trifluoroacetic acid）∶V（methanol）=42∶58 and the recoveries both reached 90%. The detection limits for both of the MCs were 0.1 μg/L after the water samples were condensed 6000 times. The detection method is simple, sensitive and fast.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
2010年第5期740-742,共3页
Chinese Journal of Analytical Chemistry
基金
国家标准物质资源共享平台建设项目(No.2005DKA21501)
北京市教委产学研项目资助
关键词
微囊藻毒素
高效液相色谱
Microcystins
High performance liquid chromatography
