摘要
目的 探讨肿瘤坏死因子(TNFα)和干扰素(IFNγ)联合应用对人肺腺癌A549细胞系的细胞毒性作用。方法 实验分为空白组、对照组、TNF组、IFN组及TNF+IFN组,应用MTT比色法检测各组的OD值,计算其抑制率。结果 TNFα和IFNγ对人肺腺癌A549细胞系均有直接抑制作用,联合用药能明显提高TNF对A549细胞系的抑制效应。结论 TNFα和IFNγ对人肺腺癌A549细胞系有明显的协同抑制效应。
Objective To explore the synergistic antitumor effect of tumor necrosis factor α(TNF α) and interferon γ (IFN γ) on human lung adenocarcinoma A549 cell line.Methods Cell growth inhibition was determined by MTT colorimetry assay. Results Both TNF α and IFN γ showed directly growth inhibition on human lung carcinoma A549 cell line. IFN γ could enhance cell growth inhibition effect of TNF α when used together.Conclusion TNF α and IFN γ displayed significant synergistic antitumor effect on human lung adenocarcinoma A549 cell line. These results give experimental evidence for the multimodality therapy of human lung cancer and other tumors.
关键词
肿瘤坏死因子
干扰素Α
协同效应
腺癌
肺肿瘤
Tumor necrosis factor α Interferon γ Synergistic effect Lung adenocarcinoma
