摘要
目的建立骨伤康复外洗颗粒的质量标准,为骨伤康复外洗颗粒的质量控制提供可行、可靠的分析方法。方法采用薄层色谱法对制剂中的黄柏进行定性鉴别;采用紫外分光光度法对制剂中君药大黄中的大黄总蒽醌进行含量测定。结果在薄层鉴别中,黄柏在供试品色谱中在与对照药材和对照品色谱相应的位置上,显相同颜色的斑点,且薄层色谱斑点清晰,而阴性对照无干扰;在含量测定中,大黄素含量在0.072～0.720mg范围内与吸光度呈良好的线性关系(r=0.9993),平均加样回收率分别为99.40%,RSD为1.91%(n=5),并确定本品中每1g含大黄以大黄总蒽醌计不得少于5.0mg。结论本方法专属性强、快速、准确、重现性好,可作为骨伤康复外洗颗粒的质量控制标准。
Objective To establish the quality standards of Gushangkangfu Wash-out pellet,and provide feasible、reliable analysis method for its quality control. Methods Phellodendron were identified by TLC method.Detemination of total anthraquinone in the main drug of the preperation by ultraviolet spectrophotometry. Results In the identification test there was the same colour speck in the preparation chromatograms as that in the reference and the TLC chromatograph spot was clear and the blank test showed no interference;The calibration curve of the absorbency was linear with the concentration of emodin in the range of 0.072~0.720 mg （r=0.9993）. The average recovery of emodin was 99.40% with RSD=1.91%（n=5 ）.The content of total anthraquinones in each 1 g including the rhubarb was not required lower than 5.0 mg. Conclusion The method is specific、fast、accurate and reproducible for the quality control of Gushangkangfu Wash-out pellet.
出处
《中国现代药物应用》
2010年第23期10-12,共3页
Chinese Journal of Modern Drug Application
关键词
骨伤康复外洗颗粒
薄层色谱法
紫外分光光度法
总蒽醌
Gushangkangfu Wash-out pellet
TLC
Ultraviolet spectrophotometry
Total anthraquinones
