摘要
以体外培养的人胚肺二倍体成纤维细胞(HELF 2BS株)为材料。当细胞培养至25代时,将细胞分为加茯苓蜂皇浆的给药组和不加茯苓蜂皇浆的对照组。2组在相同条件下传代培养,直至细胞停止增殖。结果表明:给药组细胞能生长到65代,而对照组细胞仅存活至55代。实验组细胞寿命比对照组提高18％。提示茯苓蜂皇浆对人胚肺二倍体成纤维细胞有一定的抗衰老作用。
Human embryinic lung diploid fibroblasts were used as an in vitro model for anti-ageing research in this experiment, When the cells were subcultured 25 times they were divided into two groups, group I supplemented with Poris Cocos- Royal Jelly, and group Ⅱ cultured normally as a control. The two groups were subcultured at regular intervals until the cell ceased proliferationg. If no proliferation can be found after two weeks, the cells were considered to have come to the end of their lives. The results showed that the cells of group I were subcultured for 65 population doublings, While the cells of group Ⅱ only for 55 population doublings Therefore, Poris Cocos-Royal Jelly had increased the life span of the cultureby 18%. It was shown that Poris Cocos-Royal Jelly has some anti-aging effect on the diploid fibroblasts of human embryonic lung.
关键词
茯苓蜂皇浆
成纤维细胞
衰老
Poris Cocos-Royal Jelly
fibroblasts
aging
