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百合品种IRAP指纹图谱构建 被引量：5

Construction of Fingerprinting Map for Lily by IRAP Molecular Marker
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摘要 IRAP(inter-retrotransposon amplified polymorphism)是一种基于反转录转座子的分子标记技术。文章参考烟草中的反转录转座子Tnt1的LTR保守区域设计引物,对32个百合品种进行IRAP扩增,共扩增出163条多态性带,每个品种的扩增位点数介于0～8之间,据构建的指纹图谱可将供试百合品种完全区分开。 IRAP （inter retrotransposon amplified polymorphism） is a kind of molecular markers based on retrotransposon. Primer was designed based on LTR reserve region of retrotransposon Tntl in tobacco. 32 varieties of lily were used as experimental material for IRAP amplification. Totally 163 polymorphic bands were amplified, and amplified loci quantity were 0-8 for each variety. All the 32 lily varieties could be identified by the fingerprinting map.
作者 赵福宽 张建京 夏婧 范莹莹 张克
机构地区 北京农学院生物技术系 北京农学院园林系
出处 《中国园艺文摘》 2010年第12期5-6,36,共3页 Chinese Horticulture Abstracts
基金 北京市科委"百合与特色宿根花卉种苗生产关键技术研究"(Z080005032508017)资助项目
关键词 反转录转座子 IRAP 百合 指纹图谱 retrotransposon IRAP Lily fingerprinting map
分类号 S661.1 [农业科学—果树学]
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参考文献7

  • 1Kalendar R, Grob T, Regina M, et al. IRAP and REMAP:two new retrotransposon-based DNA finger-printing techniques[J].Theor. Appl. Genet.,1999,98:704-711.
  • 2Kalendar R, Tanskanen J, Immonen S, et al. Genome evolution of wild barley(Hordeum) by BARE-1 retrotransposon dynamics in response to sharp microclimatic divergence [J].Proc. Nat. Acad. Sci. USA, 2000, 97(12):6603 6607.
  • 3Leigh F, Kalendar R, Lea V, et al. Comparison of the utility of barley retrotransposon families for genetic analysis by molecular marker techniques[J].Mol. Genet. Genom. 2003,269(4):464-474.
  • 4Bre toM P, Ruiz C, Pina J A. The diversification of Citrus clementina Hort. ex Tan,a vegetatively propagated crop species[J].Mol. Phylogenet. Evol.,2001,21(2):285-293.
  • 5Yannic G, Baumel A, Ainouche M. Uniformity of the nuclear and chloroplast genomes of Spartina maritina(Poaceae), a saltmarsh species in decline along the Western European Coast[J].Heredity, 2004,93(2):182-188.
  • 6Petr Smykal. Development of an efficient retrotransposon-based fingerprinting method for rapid pea variety identification[J].Appl. Genetic, 2006,47(3):221-230.
  • 7杜晓云,罗正荣.部分柿属植物IRAP反应体系的建立和指纹图谱构建[J].农业生物技术学报,2006,14(6):931-936. 被引量：25

二级参考文献10

  • 1任羽,王得元,张银东,李颖,王恒明.辣椒SRAP-PCR反应体系的建立与优化[J].分子植物育种,2004,2(5):689-693. 被引量：150
  • 2Bréto M P,Ruiz C,Pina J A and Asíns M J.The diversification of Citrus clementina Hort.Es Tan.,a vegetatiely propagated crop species(J).Molecular Phylogentics and Evolution,2001,21 (2):285～293
  • 3Kalendar R,Grob T and Regina M,Suoniemi A and Schulman A H.IRAP and REMAP:Two new retrotransposon based DNA fingerprinting techniques (J).Theoretical and Applied Genetics,1999,98:704～711
  • 4Kumar A and Bennetzen J L.Plant retrotransposons (J).Annual Review of Genetics,1999,33:479～532
  • 5LuoZR YonemoriKandSugiuraA.Evaluation of RAPD analysis for cultivar identification of persimmons （J）[J].日本园艺学会杂志,1995,64(3):535-541.
  • 6Manninen O,Kalendar R,Robinson J and Schulman A H.Application of BARE-1 retrotransposon markers to map a major resistance gene for net blotch in barley (J).Molecular and General Genetics,2000,264:325～334
  • 7Miller W J and Capy P.Mobile genetic elements:Protocols and genomic applications (J).Methods in Molecular Biology,2004,260:146～171
  • 8Pearce S R,Knox M,Ellis,T H N,Flavell,A J and Kumar A.Pea Ty1-copia group retrotransposons:Transpositional activity and use as markers to study genetic diversity in Pisum (J).Molecular and General Genetics,2000,263:898～907
  • 9Teo C H,Tan S H,Ho C L,Faridah Q Z,Othman Y R,Heslop-Harrison J S,Kalendar R and Schulman A H.Genome constitution and classification using retrotransposon-based markers in the orphan crop banana(J).Journal of Plant Biology,2005,48 (1):96～105
  • 10王石平,张启发.高等植物基因组中的反转录转座子[J].Acta Botanica Sinica,1998,40(4):291-297. 被引量：13

共引文献24

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二级引证文献26

中国园艺文摘
2010年 第12期

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