摘要
目的探讨加贝酯及核因子(NF-κB)在肝缺血再灌注损伤中的作用。方法采用Pringle’s法建立大鼠肝脏缺血再灌注模型,将40只SD大鼠随机分成四组,每组10只:假手术组(A组)、缺血再灌注组(B组)、加贝酯处理组(C组)、盐水对照组(D组)。光镜观察肝脏组织学变化,分别测定血浆中天冬氨酸转氨酶(AST)、丙氨酸转氨酶(ALT)及组织中髓过氧化物酶(MPO)含量;并采用SABC免疫组化法观察肝组织中NF-κB的变化。结果 :A组肝组织形态正常,无NF-κB活化,肝功能酶学和MPO正常水平;B、D组肝细胞索排列紊乱,肝小叶变形,肝细胞和内皮细胞普遍水肿变性,中央区局灶性肝细胞坏死,汇管区中性粒细胞浸润,血窦内微血栓形成,NF-kB活化最为明显,血清酶学和MPO升高最为显著(P<0.01);C组能明显减轻这种损伤,NF-κB呈中度活化。结论肝缺血再灌注时,NF-κB被活化,使中性粒细胞向组织浸润,对肝脏缺血再灌注损伤起着重要的作用。
【Objective】To investigate the effect of gabexate mesilate（GM） and NF-κB on hepatic ischemia reperfusion injury in rats.【Methods】Models of rats with hepatic ischemia reperfusion injury were established with the Pringle＇s method.Forty SD rats were randomly divided into four groups： normal control group（group A）,model group（group B）,gabexate mesilate group（group C） and saline group（group D）.Plasma alanine aminotransferase（ALT）,aspartate aminotransferase（AST） and myeloperoxidase（MPO） in liver tissue were respectively measured in each group.The activity of NF-κB was determined by sABC immunohistochemistry,also histological examination of the liver was made.【Results】In group A,the histological test,ALT,AST and MPO were almost normal,NF-kB didn＇t have activity.In group B and D,hepatocytes were arranged in turbulence,hepatic lobules were distorted,hepatocytes and endothelial cells underwent swelling or necrosis,and neutrophils infiltrated in portal area,congestion and micro-thrombosis appeared in liver sinusoids;the activity of ALT,AST,MPO and NF-kB increased remarkably（P 0.01）;but the injury was markedly decreased in rats pretreated with GM.【Conclusion】Nuclear factor kappa B is involved and may play an important role in liver ischemic reperfusion injury.
出处
《中国现代医学杂志》
CAS
CSCD
北大核心
2010年第6期821-823,共3页
China Journal of Modern Medicine
基金
湖南省科学技术厅一般项目(No:2008FJ3058)
关键词
缺血再灌注
肝脏
核因子-ΚB
加贝酯
ischemic reperfusion injury
liver
NF-κB
gabexate mesilate
