摘要
目的分析长沙地区汉族人群脑出血患者血脂水平与载脂蛋白B基因(apolipoprotein B,apoB)G12669A多态性的关系。方法收集93例散发性脑出血患者和100例正常对照者的外周血标本。采用氧化酶法测定血清甘油三酯(triglycerides,TG)、总胆固醇(total cholesterol,TC)、高密度脂蛋白(high density lipoprotein cholesterol,HDL)、低密度脂蛋白(low density lipoprotein cholesterol,LDL)水平,酶联免疫吸附法测定LP(a)浓度,免疫比浊法测定apoB100及apoAI浓度;聚合酶链式反应-限制性片段长度多态性分析法(Polymerase Chain Reaction-Restriction Fragment Length Polymorphism,PCR-RFLP)和DNA直接测序法检测apoB基因G12669A多态性。结果①长沙地区汉族人群脑出血患者的TC、TG、LDL-C和LP(a)显著高于正常对照组,而HDL-C显著降低(P<0.05);②脑出血组中,G/A基因型的TC、TG、LDL-C较之G/G型显著增高,而HDL-C则显著降低(P<0.05),其他指标无显著性差异(P>0.05);③脑出血组及对照组apoB基因G12669A多态A等位基因频率分别为0.097、0.045,脑出血组apoB基因G12669A多态A等位基因频率显著高于对照组(P<0.05)。结论长沙地区汉族人群脑出血患者的血脂水平升高,可能与apoB基因G12669A多态A等位基因存在相关性。
【Objective】To explore the effect of apolipoprotein B（apoB） gene G12669A polymorphism on plasma lipid levels of Changsha Han Chinese with cerebral hemorrhage.【Methods】Peripheral blood specimens were collected from 93 cerebral hemorrhage patients and 100 normal control people.The levels of TG,TC,HDL and LDL were examined by oxidase method.The serum levels of LP（a）,apoB-100 and apoAI were determined by immune methods.The G12669A polymorphism of apoB was analyzed by polymerase chain reaction-restriction fragment length polymorphism（PCR-PFLP） and direct sequencing.【Results】①TC,TG,LDL-C and LP（a） levels of Changsha Han Chinese with cerebral hemorrhage were significantly higher than those of control group,while the level of HDL-C was significantly lower（P0.05）.②In CH patients group,the levels of TC,TG and LDL-C in the people with G/A genotype were significantly higher than those in the people with G/G genotype,while the level of HDL-C was significantly lower（P0.05）.③A allele frequencies of apoB G12669A in cerebral hemorrhage patients and the control group were 0.097 and 0.045 respectively.A allele frequency of apoB G12669A in CH patients was significantly higher than that in the control group（P0.05）.【Conclusion】Lipid levels of cerebral hemorrhage patients in Changsha Han Chinese are significantly higher than in control group（P0.05）,which may be related with A allele of apoB G12669A polymorphism.
出处
《中国现代医学杂志》
CAS
CSCD
北大核心
2010年第2期196-200,共5页
China Journal of Modern Medicine
基金
国家自然科学基金项目(No:30600199)
关键词
载脂蛋白B
基因多态性
脑出血
血脂水平
apolipoprotein B
gene polymorphism
cerebral hemorrhage
lipid level
