摘要
为了寻求松毛虫属昆虫分类的新方法,利用COⅠ基因为分子标记针对松毛虫属(Dendromilus)10个地理种群的昆虫进行了分子系统学的研究。结果表明,COⅠ序列中,共有248个变异位点、219个简约信息位点,A+T平均含量为71.5%,大部分碱基改变为颠换。不同地理种群松毛虫的遗传距离为0.007～0.319,平均遗传距离为0.175。利用邻接法(NJ)、最大简约法(MP)、最小进化法(ME)构建了系统发育树,系统树显示10个地理种群聚为2支:梓潼、长沙、古田、玉溪、石林、禄丰6个种群聚为1支;墨江、思茅、安宁、曲靖种群聚为1支。
The mitochondrial DNA（mtDNA） cytochrome oxidase subunit Ⅰ（COⅠ） of ten geographical populations of Dendrolimus was analysed in order to explore the new classification method of the insects of Dendrolimus.Results revealed that there were 248 variable sites and 219 parsimony-informative sites in COⅠ gene fragments,and the average content of A ＋ T was about 71.5%,most of which were transformed through transversion.A molecular phylogeny for the ten geographical populations was constructed by the methods of neighbor-joining,maximum parsimony and minimum evolution.The genetic distance between different geographical populations of Dendrolimus ranged from 0.007 to 0.319,with an average of 0.175.The constructed phylogenetic tree showed that the ten geographical populations could be divided into two geographical clusters：Zitong,Changsha,Gutian,Yuxi,Shilin,and Lufeng in one cluster,and Mojiang,Simao,Anning and Qujing geographical populations in the other cluster.
出处
《东北林业大学学报》
CAS
CSCD
北大核心
2010年第11期105-107,共3页
Journal of Northeast Forestry University
基金
国家自然科学资金(31040016)
