摘要
目的探索短串联重复序列Ｄ５Ｓ４３６和Ｄ５Ｓ６５８在支气管哮喘家系连锁分析中的应用价值。方法应用聚合酶链反应（ＰＣＲ）扩增上述２个短串联重复序列，对扩增产物进行聚丙烯酰胺变性凝胶电泳检测，银染色显色分析结果。结果９２例无血缘关系的中国人中，Ｄ５Ｓ４３６检测到８个等位片段，２２种表现型，杂合率为７８％，多态信息量（ＰＩＣ）为０７６；Ｄ５Ｓ６５８检测到９个等位片段，２８种表现型，杂合率为７３％，ＰＩＣ为０８０。６个哮喘家系研究显示，哮喘相关性状（包括哮喘）与Ｄ５Ｓ４３６的最大ｌｏｄ值为２４９０（θ＝０１时）。结论Ｄ５Ｓ４３６和Ｄ５Ｓ６５８是两个较好的遗传连锁标记；连锁分析发现，在中国人中，支气管哮喘的易感基因可能在染色体５ｑ３１～３３附近。
Objective To study the clinical application value of genetic linkage analysis for bronchial asthma with two STRsD5S436 and D5S658. Methods Polymorphism of the two STRs were amplified by polymerase chain reaction, and the alleles identification were performed with denaturing polyacrylamide gel electrophoresis and silverstaining techniques. Results In 92 unrelated Chinese individuals, 8 alleles and 22 phenotypes in D5S436, 9 alleles and 28 phenotypes in D5S658 were observed. The heterozygosities were 78% and 73% respectively. The polymorphism information contents (PIC) were 076 and 0.80 respectively. Linkage analysis showed linkage of asthma related phenotypes (including asthma) with D5S436 on chromosome 5q (lod=2490, =01). ConclusionsD5S436 and D5S658 were good genetic markers. Linkage analysis demonstrates that locus on chromosome 5q3133 maybe was linked to the development of asthma in Chinese population.
出处
《中华结核和呼吸杂志》
CAS
CSCD
北大核心
1999年第7期408-410,共3页
Chinese Journal of Tuberculosis and Respiratory Diseases
基金
北京市自然科学基金
