摘要
目的：探讨二磷酸腺苷（ＡＤＰ）对细胞的促生长作用。方法：用间接免疫荧光法观察ＡＤＰ对ＢＳＣ－１细胞骨架成分的影响。结果：在用ＡＤＰ前，荧光下非洲绿猴肾上皮细胞（ＢＳＣ－１）的细胞骨架中角质蛋白纤维成分８（Ｃｙｔｏｋｅｒａｔｉｎ８，ＣＫ８）以星形位于细胞核周围，ＡＤＰ作用２ｍｉｎ后，ＣＫ８开始伸长，６ｈ后伸长到最大程度，充满整个细胞，１２ｈ后开始萎缩，２４ｈ后ＣＫ８又以星形位于细胞核的周围。而其它细胞骨架成分未见变化。结论：ＡＤＰ是肾小管上皮细胞最强的一种有丝分裂源。
Objective: Our aim was to evaluate
the role of adenosine diphosphate (ADP) on the cytoskeleton of kidney epithelial cells.
HZMethods: We used indirect immunoflurescence method to observe the influence of ADP on
the cytoskeleton of BSC1 cells. Results: In untreated confluent cells, cytokeratin 8 (CK8) was
predominantly confined to starlike spot in the area around the nucleus, but the spot began to
spread within 2 minutes after addition of ADP. This process completed at the 6th hour after
addition of ADP and a welldeveloped filamentous network throughout the cell formed. At the 12th
hour, the network began to collapse and the filaments formed a spot again in the perinuclear
area. The process completed by the 24th hour. We did not find any change of other
cytoskeleton. Conclusion: Exogenous adenosine diphosphate (ADP) was the most potent
growth mitogen for nontransformed kidney epithelial cells of BSC1 line.
出处
《中国医科大学学报》
CAS
CSCD
北大核心
1999年第3期206-207,212,共3页
Journal of China Medical University
基金
卫生部科研基金
