摘要
目的：研究ＰＧＦ２α类抗青光眼药物Ｕｎｏｐｒｏｓｔｏｎｅ对培养猴睫状肌细胞及小梁网细胞中ＭＭＰ－２表达的影响。方法：猴睫状肌及小梁网细胞融合成单层以后，暴露于不同浓度的Ｕｎｏｐｒｏｓｔｏｎｅ代谢物Ｍ１、Ｍ２或ＰＧＦ２α中，采用Ｚｙｍｏｇｒａｐｈｙ技术比较其中ＭＭＰ－２活性。结果：Ｚｙｍｏｇｒａｐｈｙ技术对睫状肌细胞培养液中ＭＭＰ－２的定量分析表明：１０－８ｍｏｌ／Ｌ，１０－７ｍｏｌ／Ｌ，１０－６ｍｏｌ／Ｌ的Ｕｎｏｐｒｏｓｔｏｎｅ代谢物Ｍ１及Ｍ２增强了ＭＭＰ－２活性表达，并且成剂量增强关系。１０－７ｍｏｌ／Ｌ，１０－６ｍｏｌ／Ｌ的Ｍ１及Ｍ２对小梁网细胞中ＭＭＰ－２表达无明显影响。结论：在通过ＭＭＰ降解细胞外基质这方面作用机理上，Ｕｎｏｐｒｏｓｔｏｎｅ可能作用于猴葡萄膜—巩膜房水流出路而对传统通路无明显影响。
Objective: Our aim was to evaluate whether matrix metall oproteinase2 (MMP2) release could be altered by exposure to PGF2 analogueUnoprostone in monkey ciliary smooth muscle and trabecular meshwork cells. Methods: After the monkey ciliary and trabecular meshwork cells became confluent, they were treated with different concentation of Unoprostone metabolites M1 or M2 or PGF2. We compared the activity of MMP2 by zymography. Results: The zymographic analysis indicated that exposure to Unoprostone metabolites M1,M2 and PGF2 increased the activity of MMP2 in monkey ciliary muscle cells, but had no effect on trabecular meshwork cells. Conclusion: These observations supported the hypothesis that increased MMP production by ciliary muscle cells played a role in increasing uveoscleral outflow facility after Unoprostone treatment in vivo.
出处
《中国医科大学学报》
CAS
CSCD
北大核心
1999年第3期189-191,共3页
Journal of China Medical University
