摘要
目的：探讨人巨细胞病毒重组抗原在ＥＬＩＳＡ中的应用。方法：采用基因工程方法组建含有巨细胞病毒ＰＰＵＬ３２及ＰＰＵＬ４４蛋白共三个强抗原决定簇基因的重组抗原克隆，以此重组抗原建立ＥＬＩＳＡ方法，用于１１７份待测血清巨细胞病毒ＩｇＭ抗体的检测，并将ＥＬＩＳＡ方法与免疫转印方法进行了比较。结果：经统计学分析，建立的ＥＬＩＳＡ方法与免疫转印检测方法的检测结果具有非常显著的一致性（χ２＝５４．０８，Ｐ＜０．０１）。与免疫转印方法比较，其敏感性和特异性分别为１００％和８１．３％；阳性预测率和阴性预测率分别为６０．５％和１００％，此ＥＬＩＳＡ方法具有良好的重复性。结论：此重组抗原具有良好的应用价值，可望替代ＨＣＭＶ感染ＥＬＩＳＡ诊断中的全病毒抗原。
Objective: Our
aim was to evaluate the application of human cytomegalovirus(HCMV) recombinant antigen in
enzymelinked immunosorbent assay(ELISA). Methods: A recombinant clone containing three
strong antigenic epitope genes of HCMV PPUL 32 and PPUL 44 was constructed by genetic
engineering method. The purified recombinant antigen was used in ELISA detect HCMV IgM
antibodies for 117 cases of serum. The ELISA method was compared with immunoblotting test.
Results: The results of the ELISA test were statistically coincident with those of immunoblotting
test(2 =54.08,P<0.01). When compared with immunoblotting test,the sensitivity and specificity
of the ELISA test were 100% and 81.3%, respectively; the positive predictive value and negative
preditive value of the ELISA test were 60.5% and 100%, respectively; meantime, the ELISA test
had a better reproducibility. T5HZConclusion: The data showed that the recombinant antigen
was very useful in ELISA test and might have a potential ability to become a substitate of
purified virion antigen in HCMV ELISA test.
出处
《中国医科大学学报》
CAS
CSCD
北大核心
1999年第3期161-163,171,共4页
Journal of China Medical University
