摘要
目的探讨依那普利对丙烯醛吸入致大鼠炎性损伤肺组织中IL-1β、IL-6表达变化的影响。方法将大鼠随机分为4组:生理盐水雾化吸入组,丙烯醛雾化吸入组,依那普利治疗组,依那普利自身对照组。用丙烯醛雾化吸入造成大鼠气道黏液高分泌炎性气道肺损伤模型。于造模及依那普利处理后1、3、6周分别用免疫组织化学染色、原位杂交、RT-PCR和蛋白免疫印迹技术检测肺组织IL-1β和IL-6蛋白及mRNA的定位与表达量。结果 IL-1β、IL-6主要分布于肺上皮细胞,胞浆染色为主。丙烯醛吸入后3周,肺组织炎症反应明显,IL-1β和IL-6的mRNA和蛋白水平均明显上调,6周达到高峰(P<0.05),而依那普利治疗下调IL-1β和IL-6mRNA和蛋白水平(P<0.05)。结论依那普利的抗炎作用可能与IL-1β和IL-6的表达下调有关。
Objective To investigate the effects of enalapril on the expressions of IL-1β and IL-6 in the lung of rats treated with aerolein inhalation. Methods Inflammatory lung injury was induced by acrolein inhalation in rats. The rats were divided into natrium solution （NS） group, acrolein group, enalapril and acroclein （EA） group, enalapril and NS （EN） group. Lungs were harvested from the rats in each group at 1 week, 3 weeks, 6 weeks after the treatment of natrium solution, aerolein inhalation and/or enalapril. The mRNA and protein expressions of IL-1β and IL-6 in the lung tissues were measured by RT-PCR, in situ hybridization, immunohistochemistry and Western blot. Results Increased immunostaining, protein level and mRNA expression of IL-1β and IL-6 were found in rat lung at 3 weeks and reached to the peak at 6 weeks post exposure to acrolein. The administration of enalapril resulted in a significant downregulation of IL-1β and IL-6 in both protein and gene level, accompany with the decrease of inflammation. Conclusion Enalapril, as a ACE inhibitor, could protect the airway from inflammation injury in acrolein-treated rats via the down-regulation of IL-1β and IL-6 expression.
出处
《四川大学学报（医学版）》
CAS
CSCD
北大核心
2010年第6期1003-1007,1038,共6页
Journal of Sichuan University(Medical Sciences)
基金
云南省自然科学基金(No.2010ZC206)资助
