摘要
采用冷凝回流提取方法和参照M38-A液体微量稀释法,对苦参、白鲜皮、土茯苓、土荆皮4种中药的乙醇提取得率及其乙醇提取物的抗真菌活性进行了研究。结果显示,苦参、白鲜皮、土茯苓和土荆皮的乙醇提取得率分别为19.54%、10.60%、14.12%和26.05%。苦参、白鲜皮、土茯苓和土荆皮的乙醇提取物对石膏小孢子菌的MIC值分别为0.7813、3.125、100和3.125mg/mL;对须癣毛癣菌的MIC值分别为0.781、3.125、50和0.781mg/mL;对犬小孢子菌的MIC值分别为3.125、1.563、25和0.781mg/mL。这4种提取物对石膏小孢子菌的MFC值分别为1.563、6.25、>100和6.25mg/mL;对须癣毛癣菌的MFC值分别为3.125、6.25、100和1.563mg/mL;对犬小孢子菌的MFC值分别为6.25、3.125、50和1.563mg/mL。结果表明,土茯苓乙醇提取物具有较差的抑菌、杀菌作用,苦参、白鲜皮和土荆皮的乙醇提取物具有明显的抑菌、杀菌作用。
To adopt reflux condense method and refer to M38,A broth microdilution,we examined the rates of alcoholic extracts of the following herbs：radix sophorae flavescentis,cortex dictamni,rhixoma smilacis glabrae and cortex pseudolaricis,and we also investigated their antifungal activities respectively.The extraction rates of the four Chinese herbs were 19.54%,10.60%,14.12% and 26.05% respectively.The minimal inhibitory concentration（MIC） of the 4 extracts against Microsporum gypseum was 0.781mg/mL,3.125mg/mL,100mg/mL and 3.125mg/mL respectively.The MIC of the 4 extracts against Trichophyton mentagrophytes was 0.781mg/mL,3.125mg/mL,50mg/mL and 0.781mg/mL respectively.The MIC of the 4 extracts against Microsporum canis was 3.125mg/mL,1.563mg/mL,25mg/mL and 0.781mg/mL respectively.The minimal fungicidal concentration（MFC） of the 4 extracts against M.gypseum was 1.563mg/mL,6.25mg/mL,100mg/mL,6.25mg/mL respectively.The MFC of the 4 extracts against T.mentagrophytes were 1.563mg/mL,6.25mg/mL,100mg/mL and 1.563mg/mL respectively.The MFC of the 4 extracts against M.canis were 6.25mg/mL,3.125mg/mL,50mg/mL and 1.563mg/mL,respectively.These results showed that the fungistasis and fungicidal effect of glabrae alcoholic ex-tract was poor and the other three alcoholic extracts showed the significant effect against the three derma-tophytes.
出处
《中国兽医科学》
CAS
CSCD
北大核心
2010年第11期1189-1193,共5页
Chinese Veterinary Science
基金
教育部"长江学者和创新团队发展计划"创新团队项目(PCSIRT0848)
宜宾市科技局科研项目(200903018)
