摘要
2010年5月从四川省某猕猴养殖场急性腹泻猕猴肠道内分离出4株致病性大肠杆菌,并对其进行了16SrDNA扩增、生化反应、血清型鉴定、药敏试验以及毒力因子基因的分子生物学检测。结果显示,该菌株血清型为O28;药敏试验证实该菌对多种抗生素具有抗性,并且呈现多重耐药性;攻毒试验证实该菌能够导致小白鼠大量死亡;多重PCR成功检测出侵袭性大肠杆菌的Einv毒力基因。确定该病原菌为肠侵袭型大肠杆菌,而且毒力很强。
Four Escherichia coli strains were isolated from Macaca mulatta with severe diarrhea on a farm in Sichuan Province in May,2010.Identification of the isolated strains was conducted by detecting 16S rDNA,biochemical reaction,serological test,drug sensitivity test and detecting toxicity gene by molecular biological method.The serotype of these bacteria was O 28 identified by serological test.In drug sensitivity test,the strains showed multidrug resistance,and they could cause mouse to death.Einv gene was detected successfully in the strains by multi-PCR.These results showed that these clinical isolates were virulent enteroinvasive E.coli.
出处
《中国兽医科学》
CAS
CSCD
北大核心
2010年第11期1106-1109,共4页
Chinese Veterinary Science
基金
教育部"长江学者和创新团队发展计划"创新团队项目(IRT0848)
关键词
肠侵袭型大肠杆菌
猕猴
毒力因子基因
分离鉴定
enteroinvasive Escherichia coli
Macaca mulatta
virulence factor gene
isolation and identification
