摘要
目的：为了改进甘氨酰脯氨酸二肽氨基肽酶同工酶的分离效果，提高肝癌特异性GPDA（GPDA-Ⅱ）对原发性肝癌的诊断价值。方法：在原阶段梯度胶电泳分离同工酶方法的基础上，将四层浓度胶（46．2g／L、77.0g／L、92.4g／L、154.0g／L）改为五层（46．2g／L、77．0g／L、92．4g／L、102．7g／L、154．0g／L）。结果：使血清PGDA-Ⅱ在PHC病人中的阳性率由原来的42．2％提高到67．6％，其阳性率与AFP、肿瘤大小无相关性。结论：进一步表明CPDA-Ⅱ正为肝癌又—新的诊断指标，对PHC有特异性诊断价值。
To improve the separative effect of glycylproline dipeptidyl aminopeptidase (GPDA)isoenzyme and raise the diagnostic value of the specific GPDA(GPDA-Ⅱ) for primary hepatic carcinoma(PHC), we modified the previously reported stage polyacryamide gel electrophoresis, four series concentraton gels (46. 2g/L, 77. 0g/L, 92. 4g/L, 154. 0g/L) were changed to five (46- 2g/L, 77. 0g/L, 92.4g/L, 102. 7g/L, 154. 0g/L). The positive rates of serum GPDA-Ⅱ in the patients with PHC wereraised from 42. 2% to 67. 6% by this method. There was no correlation between the positive rate ofGPDA-Ⅱ and AFP levels or the size of the tumors. It is suggested that GPDA-Ⅱ is a new diagnosticmark for PHC.
出处
《江苏医药》
CAS
CSCD
1999年第8期559-561,共3页
Jiangsu Medical Journal
基金
省卫生厅资助
