摘要
目的研究肠内营养对溃疡性结肠炎大鼠肠黏膜通透性的影响。方法将40只大鼠随机分为4组,正常对照组、模型对照组、甲泼尼龙组、谷氨酰胺组,后3组大鼠自由饮用5%DSS溶液7天,建立溃疡性结肠炎模型,后2组同时分别给予甲泼尼龙及谷氨酰胺干预治疗。收集大鼠24h尿液,采用高效液相色谱分析法研究大鼠肠道通透性的情况。结果 DSS诱导的溃疡性结肠炎大鼠乳果糖尿中排出率显著增高(P<0.01),甘露醇尿中排出率下降(P<0.01),L/M比值在DSS诱导的溃疡性结肠炎大鼠显著增加(P<0.01)。与DSS诱导的溃疡性结肠炎模型组比较,甲泼尼龙组大鼠及谷氨酰胺组大鼠乳果糖尿中排出率下降(P<0.01),甘露醇尿中排出率升高(P<0.01),L/M比值降低(P<0.01);谷氨酰胺组较甲泼尼龙组变化明显,但两组比较无显著差异(P>0.05)。结论溃疡性结肠炎大鼠结肠黏膜通透性增加,给予肠内营养可降低结肠黏膜通透性。
Objective To evaluate the effect of enteral nutrition on intestinal permeability of ulcerative colitis rats. Methods Forty wistar rats were divided into four groups （ control group,DSS group,methylprednisolone group and Gln group） . Rats in DSS group,methylprednisolone group and Gln group were fed with 5% DSS into the drinking water for seven days,rats in control group just drank distilled solution. The urine samples of the rats in different groups were collected respectively after test solution administrated and the rat intestinal permeability was analyzed by HPLC. Results Compared with control group,the excretion rate of lactulose in urine in DSS group significantly increased （ P 0. 01） , but the excretion rate of mannitol significantly decreased （ P 0. 01） ,and the excretion ratio of lactulose and mannitol in urine in DSS group significantly increased （ P 0. 01） . Compared with DSS group,the excretion rate of lactulose in urine decreased,but the excretion rate of mannitol increased,and the excretion ratio of lactulose and mannitol in urine decreased in methylprednisolone group and Gln group （ P 0. 01） . There was no significant difference on the excretion ratio of lactulose and mannitol in urine between methylprednisolone group and Gln group （ P 0. 05） . Conclusion The intestinal permeability of ulcerative colitis increased,but the enteral nutrition could decrease intestinal permeability of ulcerative colitis.
出处
《胃肠病学和肝病学杂志》
CAS
2010年第11期991-993,共3页
Chinese Journal of Gastroenterology and Hepatology
关键词
溃疡性结肠炎
肠内营养
高效液相色谱
肠通透性
Ulcerative colitis
Enteral nutrition
High performance liquid chromatography
Intestinal permeability
