摘要
目的观察肿瘤坏死因子相关凋亡诱导配体(TNF-related apoptosis inducing-ligand;TRAIL)对原代卵巢癌细胞生长的抑制和诱导肿瘤细胞凋亡效应,以及细胞核因子κB(NF-κB)激活和转运的影响。方法来源于12例病人原代卵巢癌细胞,给予TRAIL0、5、10、20、50、100μg/L分别作用24、48、72和96h,运用MTT比色法检测原代卵巢癌细胞生长、增殖情况;细胞免疫荧光法检测原代卵巢癌细胞凋亡和细胞内NF-κB激活-转运情况。结果 TRAIL对原代卵巢癌细胞的生长抑制呈剂量依赖性,浓度大于20μg/L时抑制率增辐减慢;浓度相同时对癌细胞的抑制呈时间依赖性,72h后抑制率上升减缓;肿瘤细胞凋亡率随TRAIL作用时间延长而增加,5μg/L24h、96h凋亡率分别是(28±1.18)%、(55±4.12)%;肿瘤细胞凋亡率也呈剂量依赖性;TRAIL作用后原代卵巢癌细胞质和胞核中NF-κB表达增加。结论 TRAIL抑制原代卵巢癌细胞的增殖和生长,并诱导癌细胞的凋亡;激活癌细胞质中NF-κB向细胞核内转运。
Objective To investigate the regulatory effects of TRAIL on proliferation and apoptosis of primary human ovarian cancer cell in vitro,and the activation and transportation of NF-κB.Methods Primary human ovarian cancer cells were obtained from twelve patients.Each patient＇s cell was divided to six groups,and treated with TRAIL（5～100μg/L） for 24～96h respectively.The ratio of growth inhibition was measured with MTT in primary human ovarian cancer cells.The ratio of apoptosis and the transportation and activation of NF-κB were measured by immunofluorescence in primary human ovarian cancer cells.Results The ratio of growth inhibition increased in dose-and time-dependent manner when the concentration was 5,10or 20μg/L at 24,48,72h.The ratio of apoptosis increased along with the time lasting.It was（28±1.18）% with concentration of 100μg/L at 24h,and（55± 4.12）%at 96h.The level of NF-κB in nucleus and cytoplasm was highly expressed.Conclusion TRAIL inhibits proliferation and growth of primary human ovarian cancer cell,and induces apoptosis.It enhances conveying the NF-κB from cytolymph to nucleolus.
出处
《肿瘤防治研究》
CAS
CSCD
北大核心
2010年第11期1296-1299,共4页
Cancer Research on Prevention and Treatment
