摘要
目的研究皮层肌动蛋白(cortactin)基因CTTN在原发性肝癌和正常肝组织中的表达情况,探讨CTTN表达与肿瘤侵袭和转移能力之间的关系。方法提取人肝癌和肝血管瘤周围正常肝组织总RNA,RT-PCR扩增CTTN和GAPDH基因片段并构建pMD18-T(+)/CTTN和pMD18-T(+)/GAPDH重组质粒。采用实时荧光定量PCR双标准曲线法检测CTTN mRNA表达量,分析CTTN表达差异。结果原发性肝癌高侵袭组CTTN表达明显高于低侵袭组和对照组[(16.791+10.693)vs.(7.279+4.414),(6.436+4.618)],差异有统计学意义(P<0.01);低侵袭组与对照组比较差异无统计学意义(P>0.05)。结论 CTTN基因表达水平与原发性肝癌的侵袭和转移能力有关,CTTN高表达可能是原发性肝癌侵袭力增强的因素之一。
Objective To investigate the relationship of cortactin gene（CTTN）level with invasion and metastasis in primary hepatic carcinoma.Methods Total RNA was extracted from human liver cancer tissues and normal hepatic tissues adjacent to cavernous hemangiomas of liver.To build standard curves,CTTN and GAPDH gene were amplified by RT-PCR and pMD18-T（＋）/CTTN and pMD18T（＋）/GAPDH recombinant plasmid were established and confirmed as expected.The expression of CTTN was detected by real-time fluorescent quantitative PCR and the relative values were analyzed with specimens grouped according to their invasive power.Results The mRNA level of CTTN was significantly higher in high invasive liver cancer tissues than that in low invasive liver cancer tissues and noncancerous tissues[（16.791＋10.693）vs.（7.279＋4.414）,（6.436＋4.618）]（P〈0.01）.There was no statistical significance between low invasive liver cancer tissues and noncancerous tissues（P〈0.05）.Conclusion The increase of CTTN expression contribute to invasion and metastasis in primary hepatic carcinoma and CTTN overexpression may activate tumor cells to become more invasive.
出处
《肿瘤防治研究》
CAS
CSCD
北大核心
2010年第11期1285-1287,共3页
Cancer Research on Prevention and Treatment
基金
空军总医院科研课题资助项目(KZ2007001)
关键词
皮层肌动蛋白
癌
肝细胞
基因表达
聚合酶链式反应
肿瘤侵袭
Cortactin
Carcinoma
hepatocellular
Gene expression
Polymerase chain reaction
Neoplasm invasion
