摘要
目的研究人参皂苷Rg3(ginsenoside Rg3)对人鼻咽低分化鳞癌HNE-1细胞的增殖、迁移及体外血管生成的影响。方法用不同浓度人参皂苷Rg3处理HNE-1细胞,MTT法检测HNE-1细胞增殖活性;创伤修复实验检测细胞迁移能力;观察HNE-1细胞能否在Matrigel上形成血管网状结构及其特点;体外管道形成抑制试验检测不同浓度人参皂苷Rg3对HNE-1细胞管道形成能力的影响。结果不同浓度人参皂苷Rg3(50、100、200μg/ml)对HNE-1、CRL-2480细胞均有一定的增殖抑制作用,呈浓度依赖性趋势,无时间依赖性,差异均无统计学意义(P>0.05);人参皂苷Rg3(25、50、100、200μg/ml)可以显著降低HNE-1细胞迁移速度,与对照组比较差异有统计学意义(P<0.001),与浓度呈负相关(r=-0.964;P<0.001);HNE-1细胞在Matrigel上培养能形成血管网状样结构;人参皂苷Rg3能抑制HNE-1细胞体外管道形成(P<0.01),其管状结构数量与人参皂苷Rg3浓度呈负相关(r=-0.928;P<0.01)。结论人参皂苷Rg3有一定的抗HNE-1细胞增殖作用;HNE-1细胞具有血管生成拟态;人参皂苷Rg3能够抑制HNE-1细胞的迁移和体外血管生成拟态的形成。
Objective To study the inhibitory effects of Ginsenoside Rg3on proliferation,migration and in vitro angiogenesis of human nasopharyngeal carcinoma HNE-1 cells.Methods The proliferative activity,migration and tube-like structures（TLSs） formation of HNE-1 cells,cultured in medium with different concentration of Ginsenoside Rg3,were determined by MTT assay,wound healing assay and in vitro anti-angiogenic test,respectively.Anti-angiogenic test was based on preliminary experiment on the observation of TLSs formed by HNE-1cells on Matrigel and their structural characteristics.Results Ginsenoside Rg3 had anti-proliferative effect both in HNE-1and CRL-2480 cells at the concentrations of 50,100 and 200μg/ml,which was dose-dependent but not time-dependent,and neither of them has statistical significance（P〈0.05）.Ginsenoside Rg3could significantly decrease the migration velocity of HNE-1cells at the concentrations of 25,50,100 and 200μg/ml,of which the differences were all statistically significant compared with control group（P〈0.01）,and the effect was negatively correlated with the concentration of Ginsenoside Rg3（r=-0.964;P〈0.001）.HNE-1 cells could form TLSs on matrigel.Ginsenoside Rg3could inhibit the formation of TLSs of HNE-1 cells,and the number of tubule was negatively correlated with the concentration of Ginsenoside Rg3（r=-0.928;P〈0.01）.Conclusion Ginsenoside Rg3has certain anti-proliferative effect on HNE-1cells.HNE-1 cells could form TLSs on matrigel.Ginsenoside Rg3 can inhibit the migration and vasculogenic mimicry of HNE-1 cells.
出处
《肿瘤防治研究》
CAS
CSCD
北大核心
2010年第11期1226-1229,共4页
Cancer Research on Prevention and Treatment
基金
广东省中医药管理局资助课题(1040101)
关键词
鼻咽癌
血管生成拟态
人参皂苷RG3
Human nasopharyngeal carcinoma（NPC）
Vasculogenic mimicry
Ginsenoside Rg3
