摘要
目的:观察麝香保心丸(SXBXW)对血管平滑肌细胞表型转化的影响。方法:以血管平滑肌细胞系RASMCP8为模型,设对照组,SXBXW0.25、0.5、1.0和2.0g/L组,经不同浓度的SXBXW作用后,用流式细胞术测定RASMCP8细胞的收缩型特异性分子标志物α-平滑肌肌动蛋白(α-SMA)和平滑肌肌球蛋白重链(SM-MHC)表达的变化。结果:与对照组相比,经SXBXW处理后,α-SMA和SM-MHC的阴性细胞百分比降低,阳性细胞百分比增加。结论:SXBXW能促使血管平滑肌细胞的表型从合成型向收缩型转化。
AIM： To observe the effects of She xiang bao xin wan （SXBXW） of Chinese patent drug on the phenotype transformation of vascular smooth muscle cells.METHODS： The vascular smooth muscle cell line RASMC P8 was used as the cell model in this experiment. The cells were treated with SXBXW at the doses of 0.25 g/L, 0.50 g/L, 1.0 g/L and 2.0 g/L, respectively, and control cells were treated with the same volume of culture medium without SXBXW. The expression of α-smooth muscle actin （α-SMA） and the smooth muscle myosin heavy chain （SM-MHC）, which served as specific molecular markers of vascular smooth muscle contraction in RASMC P8 cells, was analyzed by flow cytometry. RESULTS： The percentages of α-SMA and SM-MHC negative cells in SXBXW treated groups were lower than those in control group. Meanwhile, the percentages of α-SMA and SM-MHC positive cells were increased in RASMC P8 cells treated with SXBXW, indicating that SXBXW prompted the transformation of RASMC P8 cells from synthetic to contractile phenotype.CONCLUSION： Chinese patent drug SXBXW plays a role in transforming the cell phenotype from synthetic to the contraction in vascular smooth muscle.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2010年第11期2149-2154,共6页
Chinese Journal of Pathophysiology
基金
浙江省中医药管理局资助课题(No.2007CA096)
关键词
麝香保心丸
血管平滑肌细胞
表型
She xiang bao xin wan Vascular smooth muscle cells Phenotype
